Repeated cultivations (4 passages) of salmonellae (18 strains) resistant to cadmium, streptomycin and beta-lactam antibiotics in Müller-Hinton Broth (MHB) and Mac-Conkey Broth (MCB) without and with CdSO4 (2, 20 and 100 mg/L) showed a higher toxic effect of cadmium in MCB. The strains survived at CdSO4 100 mg/L in MHB for four transfers, in MCB only a single transfer. In dependence on the medium used and amount of metal added, the increase of resistance to antibiotics was different. In MHB, the same levels of resistance to carbenicillin and streptomycin were induced by CdSO4 (20 and 100 mg/L), in MCB it was by 2 and 20 mg/L. Simultaneous stop of the growth of a control culture S. typhimurium with chromosomal resistance to streptomycin, isolates with and without plasmid in MCB which contained CdSO4 100 mg/L, and the results of conjugal transfer of resistance suggest that changes of resistance to antibiotics were not mediated by determinants of resistance to antibiotics. The binding of cadmium to outer membrane protein can cause a decreased permeability to these antibiotics as a resistance mechanism.

• MeSH
• antibakteriální látky farmakologie   MeSH
• antibiotická rezistence * genetika   MeSH
• Escherichia coli účinky léků   genetika   MeSH
• kadmium farmakologie   MeSH
• konjugace genetická MeSH
• laktamy MeSH
• mnohočetná léková rezistence genetika   MeSH
• prasata MeSH
• R-plasmidy genetika   MeSH
• Salmonella účinky léků   genetika   izolace a purifikace   MeSH
• streptomycin farmakologie   MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• kadmium MeSH
• laktamy MeSH
• streptomycin MeSH