Common procedure of isolation of isoquinoline alkaloids and the novel separation techniques are discussed and exemplified. The methods used for determination of isoquinoline alkaloids are reviewed, including GC, HPLC, TLC, immunochemical methods and some special techniques. The selected examples of applications are included.

• MeSH
• alkaloidy analýza   izolace a purifikace   MeSH
• chromatografie metody   MeSH
• isochinoliny analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• alkaloidy MeSH
• isochinoliny MeSH

Three new alkaloids, bersavine (3), muraricine (4), and berbostrejdine (8), together with seven known isoquinoline alkaloids (1-2, 5-7, 9, and 10) were isolated from an alkaloidal extract of the root bark of Berberis vulgaris. The structures of the isolated compounds were determined by spectroscopic methods, including 1D and 2D NMR techniques, HRMS, and optical rotation, and by comparison of the obtained data with those in the literature. The NMR data of berbamine (5), aromoline (6), and obamegine (7) were completely assigned employing 2D NMR experiments. Alkaloids isolated in sufficient amounts were evaluated for their in vitro acetylcholinesterase, butyrylcholinesterase (BuChE), prolyl oligopeptidase, and glycogen synthase kinase-3β inhibitory activities. Selected compounds were studied for their ability to permeate through the blood-brain barrier. Significant human BuChE ( hBuChE) inhibitory activity was demonstrated by 6 (IC50 = 0.82 ± 0.10 μM). The in vitro data were further supported by computational analysis that showed the accommodation of 6 in the active site of hBuChE.

• MeSH
• acetylcholinesterasa metabolismus   MeSH
• alkaloidy chemie   izolace a purifikace   terapeutické užití   MeSH
• Alzheimerova nemoc farmakoterapie   MeSH
• Berberis chemie   MeSH
• butyrylcholinesterasa metabolismus   MeSH
• cholinesterasové inhibitory terapeutické užití   MeSH
• hematoencefalická bariéra účinky léků   MeSH
• isochinoliny chemie   izolace a purifikace   terapeutické užití   MeSH
• lidé MeSH
• magnetická rezonanční spektroskopie MeSH
• rostlinné exsudáty analýza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• acetylcholinesterasa MeSH
• alkaloidy MeSH
• butyrylcholinesterasa MeSH
• cholinesterasové inhibitory MeSH
• isochinoliny MeSH
• rostlinné exsudáty MeSH