Reduction of dietary obesity in aP2-Ucp transgenic mice mechanism and adipose tissue morphology Dotaz Zobrazit nápovědu
C57BL6/J mice with the expression of the mitochondrial uncoupling protein (UCP) gene from the fat-specific aP2 gene promoter were used to study the mechanism by which the aP2-Ucp transgene affects adiposity and reduces high-fat diet induced obesity. In the transgenic mice, UCP synthesized in white fat was inserted into mitochondria, and oxygen uptake by epididymal fat fragments indicated UCP-induced thermogenesis. The respirometry data, UCP content, cytochrome oxidase activity, and tissue morphology suggested functional involution of brown fat. Despite 25- to 50-fold lower mitochondrial cytochrome oxidase activity in white than in brown fat cells, total oxidative capacity in white and brown adipose tissue is comparable. Appearance of novel small cells in the gonadal fat of the transgenic mice was associated with a higher DNA content than that of the nontransgenic mice. The results prove a potential of transgenically altered mitochondria in white fat to modulate adiposity and energy expenditure and suggest the existence of a yet unidentified site-specific link between energy metabolism in adipocytes and cellularity.
- MeSH
- adaptorové proteiny vezikulární transportní MeSH
- adaptorový proteinový komplex - alfa-podjednotky MeSH
- adaptorový proteinový komplex 2 MeSH
- antigeny analýza MeSH
- dietní tuky MeSH
- dýchání MeSH
- hnědá tuková tkáň enzymologie MeSH
- iontové kanály MeSH
- lipoproteinlipasa metabolismus MeSH
- membránové proteiny genetika imunologie metabolismus MeSH
- messenger RNA metabolismus MeSH
- mitochondriální proteiny MeSH
- myši inbrední C57BL MeSH
- myši transgenní genetika MeSH
- myši MeSH
- obezita genetika patologie patofyziologie MeSH
- respirační komplex IV imunologie metabolismus MeSH
- transportní proteiny genetika imunologie metabolismus MeSH
- tuková tkáň enzymologie patologie MeSH
- uncoupling protein 1 MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Názvy látek
- adaptorové proteiny vezikulární transportní MeSH
- adaptorový proteinový komplex - alfa-podjednotky MeSH
- adaptorový proteinový komplex 2 MeSH
- antigeny MeSH
- dietní tuky MeSH
- iontové kanály MeSH
- lipoproteinlipasa MeSH
- membránové proteiny MeSH
- messenger RNA MeSH
- mitochondriální proteiny MeSH
- respirační komplex IV MeSH
- transportní proteiny MeSH
- uncoupling protein 1 MeSH
