beta-Catenin plays a pivotal role in Wnt signaling during embryogenesis and is a component of adherens junctions. Since targeted disruption of the beta-catenin gene is lethal at gastrulation we have used a D6-Cre mouse line for conditional inactivation of beta-catenin in the mouse cerebral cortex and hippocampus after embryonic day (E) 10.5. In D6-Cre floxed beta-catenin mice, hippocampal CA1-CA2 fields are disrupted in similar manner as in Wnt-3a and LEF-1 mutants. The cortex of D6-Cre floxed beta-catenin mutants is strongly affected which contrasts with the normal cortex observed in Wnt-3a and LEF-1 mutants. Severe abnormalities in the organization of the neuroepithelium are observed that include disrupted interkinetic nuclear migration, loss of adherens junctions, impaired radial migration of neurons toward superficial layers and decreased cell proliferation after E15.5. At newborn stage, a premature disassembly of the radial glial scaffold and increased numbers of astrocytes are found in the cortex.
- MeSH
- beta Catenin MeSH
- Cytoskeletal Proteins genetics metabolism MeSH
- DNA-Binding Proteins metabolism MeSH
- Hippocampus embryology growth & development MeSH
- In Situ Hybridization MeSH
- Immunohistochemistry MeSH
- Mitosis MeSH
- Cerebral Cortex embryology growth & development MeSH
- Mice, Inbred C57BL MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Neuroglia metabolism MeSH
- Neurons metabolism MeSH
- Cell Movement * MeSH
- Trans-Activators genetics metabolism MeSH
- Transcription Factors genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta Catenin MeSH
- CTNNB1 protein, mouse MeSH Browser
- Cytoskeletal Proteins MeSH
- DNA-Binding Proteins MeSH
- Trans-Activators MeSH
- Transcription Factors MeSH
