SOAP
Dotaz
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The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights Mw of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.
- Klíčová slova
- Downstream process, Isolation, P3HB, PHA, Recovery,
- MeSH
- biomasa MeSH
- bioreaktory * MeSH
- hydroxybutyráty MeSH
- kyselina 3-hydroxymáselná MeSH
- mýdla * MeSH
- polyestery MeSH
- vaření MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hydroxybutyráty MeSH
- kyselina 3-hydroxymáselná MeSH
- mýdla * MeSH
- poly-beta-hydroxybutyrate MeSH Prohlížeč
- polyestery MeSH
- Klíčová slova
- INFANT CARE *, SKIN *,
- MeSH
- dítě MeSH
- kojenec MeSH
- kožní nemoci * MeSH
- kůže * MeSH
- lidé MeSH
- mýdla * MeSH
- novorozenec MeSH
- péče o kojence * MeSH
- voda * MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mýdla * MeSH
- voda * MeSH
- Klíčová slova
- DDT *, PEDICULOSIS *, SOAPS *,
- MeSH
- DDT * MeSH
- mýdla * MeSH
- zavšivení * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DDT * MeSH
- mýdla * MeSH
- Klíčová slova
- DERMATITIS, CONTACT *, SKIN *, SOAPS *,
- MeSH
- dermatitida * MeSH
- kontaktní dermatitida * MeSH
- kůže * MeSH
- lidé MeSH
- mýdla * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mýdla * MeSH
- MeSH
- Bacteria účinky léků MeSH
- dezinficiencia farmakologie MeSH
- mýdla farmakologie MeSH
- salicylamidy farmakologie MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- dezinficiencia MeSH
- mýdla MeSH
- salicylamidy MeSH
- MeSH
- Bacteria účinky léků MeSH
- časové faktory MeSH
- hexachlorofen * MeSH
- lidé MeSH
- mýdla normy MeSH
- předoperační péče normy MeSH
- ruka mikrobiologie MeSH
- sterilizace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hexachlorofen * MeSH
- mýdla MeSH
- Klíčová slova
- ANTI-INFECTIVE AGENTS, LOCAL *, HEXACHLOROPHENE *, SOAPS *,
- MeSH
- antiinfekční látky lokální * MeSH
- antiinfekční látky * MeSH
- dezinficiencia * MeSH
- hexachlorofen * MeSH
- lidé MeSH
- mýdla * MeSH
- sterilizace * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antiinfekční látky lokální * MeSH
- antiinfekční látky * MeSH
- dezinficiencia * MeSH
- hexachlorofen * MeSH
- mýdla * MeSH
Long-chain lead carboxylates, on the one hand, represent compounds for versatile industrial applications in high-tech industries, while on the other hand, they are predominant constituents of secondary products of saponification of paint layers in works of art. Affecting significantly the appearance and stability of painted works of art, saponification is one of the most serious problems of preservation of cultural heritage objects. Despite their versatility as well as hazardousness, there is a paucity of single-crystal X-ray structures of long-chain carboxylates, due to difficulties in preparing single crystals of sufficient quality. We studied the crystal structure of polycrystalline mixed lead carboxylates of the formula Pb(C16)2-x(C18)x (x = 0; 0.25; 0.5; 0.75; 1; 1.5; 2), where C16 and C18 stand for hexadecanoate (palmitate) and octadecanoate (stearate) anions, respectively, by complementary X-ray powder diffraction (XRPD) and 13C and 207Pb solid state NMR (ssNMR). Mixed lead carboxylates consisting of hexadecanoate and octadecanoate are relevant to the formation of soaps in egg yolk and/or oil-based binders combined with lead-based pigments, which belong to the most common pigments in history. Combining an advanced XRPD analysis with a comparative analysis of ssNMR parameters, we described the structural model of mixed lead carboxylates. We revealed that both hexadecanoate (C16) and octadecanoate (C18) chains are present in one crystal structure, creating the statistical disorder at the ethyl end of the chains. Based on the 207Pb ssNMR spectra, we revealed two distinct local environments of lead atoms, corresponding to the symmetrically (i.e., (C16)-Pb-(C16) and/or (C18)-Pb-(C18) and asymmetrically (i.e., (C16)-Pb-(C18)) substituted lead carboxylates, and we confirmed the formation of a holo-directed structure for both the structural motifs. The structural models were applied to identify the neo-formed crystalline lead soap in a model experiment simulating the simplified historic paint consisting of the pigment lead tin yellow type I and emulsion binder prepared from egg yolk and linseed oil. We identified the secondary product as a mixed lead carboxylate of the composition Pb(C16)(C18).
- Publikační typ
- časopisecké články MeSH
- MeSH
- hexachlorofen farmakologie MeSH
- lidé MeSH
- mýdla farmakologie MeSH
- ruka mikrobiologie MeSH
- sterilizace účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hexachlorofen MeSH
- mýdla MeSH
- MeSH
- Bacteria účinky léků MeSH
- chirurgie operační MeSH
- dezinficiencia * MeSH
- hexachlorofen farmakologie MeSH
- kůže účinky léků MeSH
- lidé MeSH
- mýdla * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dezinficiencia * MeSH
- hexachlorofen MeSH
- mýdla * MeSH
