• Klíčová slova
• PROTEASES/metabolism *, STREPTOMYCES *,
• MeSH
• endopeptidasy * MeSH
• hydrolasy * MeSH
• proteasy metabolismus   MeSH
• Streptomyces griseus * MeSH
• Streptomyces * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• endopeptidasy * MeSH
• hydrolasy * MeSH
• proteasy MeSH

• Klíčová slova
• EXPERIMENTAL LAB STUDY *, FERMENTATION *, PYRIDOXINE *, STREPTOMYCES *,
• MeSH
• fermentace * MeSH
• mycelium * MeSH
• pyridoxin * MeSH
• Streptomyces aureofaciens * MeSH
• Streptomyces * MeSH
• vitamin B6 * MeSH
• výzkum * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• pyridoxin * MeSH
• vitamin B6 * MeSH

σ factors are considered as positive regulators of gene expression. Here we reveal the opposite, inhibitory role of these proteins. We used a combination of molecular biology methods and computational modeling to analyze the regulatory activity of the extracytoplasmic σE factor from Streptomyces coelicolor. The direct activator/repressor function of σE was then explored by experimental analysis of selected promoter regions in vivo. Additionally, the σE interactome was defined. Taken together, the results characterize σE, its regulation, regulon, and suggest its direct inhibitory function (as a repressor) in gene expression, a phenomenon that may be common also to other σ factors and organisms.

• MeSH
• počítačová simulace MeSH
• sigma faktor genetika   MeSH
• Streptomyces coelicolor * genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• sigma faktor MeSH

• Klíčová slova
• CHLORTETRACYCLINE *, CULTURE MEDIA *, EXPERIMENTAL LAB STUDY *, METABOLISM *, STARCH *, STREPTOMYCES *,
• MeSH
• chlortetracyklin * MeSH
• kultivační média * MeSH
• metabolismus * MeSH
• škrob * MeSH
• Streptomyces aureofaciens * MeSH
• Streptomyces * MeSH
• výzkum * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chlortetracyklin * MeSH
• kultivační média * MeSH
• škrob * MeSH

• Klíčová slova
• STREPTOMYCES *,
• MeSH
• Streptomyces griseus * MeSH
• Streptomyces * MeSH
• Publikační typ
• časopisecké články MeSH

The small bacterial laccase from the actinobacterium Streptomyces coelicolor which lacks the second of the three domains of the laccases structurally characterized to date was crystallized. This multi-copper phenol oxidase crystallizes in a primitive tetragonal lattice, with unit-cell parameters a = b = 179.8, c = 175.3 A. The crystals belong to either space group P4(1)2(1)2 or P4(3)2(1)2. The self-rotation function shows the presence of a noncrystallographic threefold axis in the structure. Phases will be determined from the anomalous signal of the natively present copper ions.

• MeSH
• difrakce rentgenového záření MeSH
• krystalizace MeSH
• lakasa chemie   genetika   metabolismus   MeSH
• molekulová hmotnost MeSH
• Streptomyces coelicolor enzymologie   genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• lakasa MeSH

BACKGROUND: The first systematic study of small non-coding RNAs (sRNA, ncRNA) in Streptomyces is presented. Except for a few exceptions, the Streptomyces sRNAs, as well as the sRNAs in other genera of the Actinomyces group, have remained unstudied. This study was based on sequence conservation in intergenic regions of Streptomyces, localization of transcription termination factors, and genomic arrangement of genes flanking the predicted sRNAs. RESULTS: Thirty-two potential sRNAs in Streptomyces were predicted. Of these, expression of 20 was detected by microarrays and RT-PCR. The prediction was validated by a structure based computational approach. Two predicted sRNAs were found to be terminated by transcription termination factors different from the Rho-independent terminators. One predicted sRNA was identified computationally with high probability as a Streptomyces 6S RNA. Out of the 32 predicted sRNAs, 24 were found to be structurally dissimilar from known sRNAs. CONCLUSION: Streptomyces is the largest genus of Actinomyces, whose sRNAs have not been studied. The Actinomyces is a group of bacterial species with unique genomes and phenotypes. Therefore, in Actinomyces, new unique bacterial sRNAs may be identified. The sequence and structural dissimilarity of the predicted Streptomyces sRNAs demonstrated by this study serve as the first evidence of the uniqueness of Actinomyces sRNAs.

• MeSH
• algoritmy MeSH
• bakteriální RNA chemie   genetika   MeSH
• druhová specificita MeSH
• genom bakteriální MeSH
• intergenová DNA MeSH
• konformace nukleové kyseliny MeSH
• molekulární modely MeSH
• nekódující RNA chemie   genetika   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• sekvence nukleotidů MeSH
• sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
• Streptomyces coelicolor genetika   MeSH
• Streptomyces genetika   MeSH
• terminátorové oblasti (genetika) MeSH
• výpočetní biologie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• validační studie MeSH
• Názvy látek
• bakteriální RNA MeSH
• intergenová DNA MeSH
• nekódující RNA MeSH

In vitro phosphorylation reaction using extracts prepared from cells in the exponential phase of growth and aerial spores of Streptomyces coelicolor displayed the presence of multiply phosphorylated proteins. Effect of protein kinase inhibitors (PKIs) (geldanamycin, wortmannin, apigenin, genistein, roscovitine, methyl 2,5-dihydroxycinnamate, rapamycin, staurosporine) was determined on protein phosphorylation and on germination of spores. The in vitro experiments showed differences in phosphoprotein pattern due to the presence of PKIs. Cultivation of aerial spores with PKIs led to a significant delay in germ tube emergence and filament formation. However, none of the tested PKIs completely blocked the germination process. These results indicate that protein kinases of spores form complex networks sharing common modulating site that plays an important role in proper timing of early developmental events.

• MeSH
• bakteriální proteiny metabolismus   MeSH
• fosforylace účinky léků   MeSH
• inhibitory proteinkinas farmakologie   MeSH
• proteinkinasy účinky léků   metabolismus   MeSH
• spory bakteriální účinky léků   metabolismus   MeSH
• Streptomyces coelicolor účinky léků   enzymologie   růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• inhibitory proteinkinas MeSH
• proteinkinasy MeSH

Streptomyces coelicolor genome carries two apparently paralogous genes, SCO4164 and SCO5854, that encode putative thiosulfate sulfurtransferases (rhodaneses). These genes (and their presumed translation products) are highly conserved and widely distributed across actinobacterial genomes. The SCO4164 knockout strain was unable to grow on minimal media with either sulfate or sulfite as the sole sulfur source. The SCO5854 mutant had no growth defects in the presence of various sulfur sources; however, it produced significantly less amounts of actinorhodin. Furthermore, we discuss possible links between basic interconversions of inorganic sulfur species and secondary metabolism in S. coelicolor.

• MeSH
• anthrachinony metabolismus   MeSH
• antibakteriální látky metabolismus   MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• kultivační média metabolismus   MeSH
• sekundární metabolismus MeSH
• sírany metabolismus   MeSH
• Streptomyces coelicolor enzymologie   genetika   růst a vývoj   metabolismus   MeSH
• thiosulfátsulfurtransferasa genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• actinorhodin MeSH Prohlížeč
• anthrachinony MeSH
• antibakteriální látky MeSH
• bakteriální proteiny MeSH
• kultivační média MeSH
• sírany MeSH
• thiosulfátsulfurtransferasa MeSH

• Klíčová slova
• PANTOTHENIC ACID *, STREPTOMYCES *,
• MeSH
• kyselina pantothenová * MeSH
• lidé MeSH
• mycelium * MeSH
• Streptomyces * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kyselina pantothenová * MeSH