Many experiments have demonstrated that some cell lines are resistant to chemically induced apoptosis in vitro, and that apoptosis itself is far from being a homogenous phenomenon. Here we show that 10 microg/ml etoposide elicited only minor changes in Bowes human melanoma cells (temporary decrease in cell viability and proliferation, transient phospatidylserine externalization and caspase-3 activation), which weren't clearly capable to start apoptotic pathway in the entire treated population. On the other hand, potassium chromate at concentration of 150 microg/ml executed cell death bearing some features of apoptosis (cell blebbing, caspase-3 activation and cytoskeletal changes) but lacking or showing weakly others (DNA fragmentation and phospatidylserine externalization). Our results suggest that in detecting apoptosis several fault-proof detection systems are to be used to avoid misleading results and conclusions in each experimental setting.
- MeSH
- Actins metabolism MeSH
- Annexin A5 analysis MeSH
- Apoptosis * drug effects MeSH
- Biomarkers analysis MeSH
- Cell Division MeSH
- Chromates pharmacology MeSH
- DNA, Neoplasm analysis drug effects MeSH
- Etoposide pharmacology MeSH
- Fluorescein-5-isothiocyanate MeSH
- Fluorescent Dyes MeSH
- Phosphatidylserines analysis MeSH
- DNA Fragmentation drug effects MeSH
- Caspase 3 MeSH
- Caspases metabolism MeSH
- Humans MeSH
- Melanoma, Experimental chemistry pathology MeSH
- Tumor Cells, Cultured chemistry pathology MeSH
- Potassium Compounds pharmacology MeSH
- Microscopy, Video MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Annexin A5 MeSH
- Biomarkers MeSH
- CASP3 protein, human MeSH Browser
- Chromates MeSH
- DNA, Neoplasm MeSH
- Etoposide MeSH
- Fluorescein-5-isothiocyanate MeSH
- Fluorescent Dyes MeSH
- Phosphatidylserines MeSH
- Caspase 3 MeSH
- Caspases MeSH
- potassium chromate(VI) MeSH Browser
- Potassium Compounds MeSH
