In this study, we conducted an extensive investigation of the biodegradation capabilities and stress response of the newly isolated strain Pseudomonas veronii SM-20 in order, to assess its potential for bioremediation of sites contaminated with polycyclic aromatic hydrocarbons (PAHs). Initially, phenotype microarray technology demonstrated the strain's proficiency in utilizing various carbon sources and its resistance to certain stressors. Genomic analysis has identified numerous genes involved in aromatic hydrocarbon metabolism. Biodegradation assay analyzed the depletion of phenanthrene (PHE) when it was added as a sole carbon and energy source. We found that P. veronii strain SM-20 degraded approximately 25% of PHE over a 30-day period, starting with an initial concentration of 600 µg/mL, while being utilized for growth. The degradation process involved PHE oxidation to an unstable arene oxide and 9,10-phenanthrenequinone, followed by ring-cleavage. Comparative proteomics provided a comprehensive understanding of how the entire proteome responded to PHE exposure, revealing the strain's adaptation in terms of aromatic metabolism, surface properties, and defense mechanism. In conclusion, our findings shed light on the promising attributes of P. veronii SM-20 and offer valuable insights for the use of P. veronii species in environmental restoration efforts targeting PAH-impacted sites.

• Klíčová slova
• Pseudomonas, biodegradation, genomics, phenanthrene (PHE), polycyclic aromatic hydrocarbons (PAHs), proteomics,
• Publikační typ
• časopisecké články MeSH

The study is focused on artificial soil which is supposed to be a standardized "soil like" medium. We compared physico-chemical properties and extractability of Phenanthrene from 25 artificial soils prepared according to OECD standardized procedures at different laboratories. A substantial range of soil properties was found, also for parameters which should be standardized because they have an important influence on the bioavailability of pollutants (e.g. total organic carbon ranged from 1.4 to 6.1%). The extractability of Phe was measured by supercritical fluid extraction (SFE) at harsh and mild conditions. Highly variable Phe extractability from different soils (3-89%) was observed. The extractability was strongly related (R(2)=0.87) to total organic carbon content, 0.1-2mm particle size, and humic/fulvic acid ratio in the following multiple regression model: SFE (%)=1.35*sand (%)-0.77*TOC (%)2+0.27*HA/FA.

• MeSH
• adsorpce MeSH
• fenantreny analýza   chemie   MeSH
• látky znečišťující půdu analýza   chemie   MeSH
• půda chemie   MeSH
• superkritická fluidní chromatografie * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• fenantreny MeSH
• látky znečišťující půdu MeSH
• phenanthrene MeSH Prohlížeč
• půda MeSH

The effect of phenanthrene (Phe) on induction of ethoxyresorufinO-deethylase (EROD) activity and oxidative stress was examined in immune organs of yellowfin seabream Acanthopagrus latus. Fish were treated with a single intraperitoneal injection at 2, 20, or 40 mg kg-1. The Phe concentration in spleen, EROD activity, superoxide dismutase (SOD) and catalase (CAT) activity, ascorbic acid (AsA), total glutathione (GSH), lipid peroxidation (LPO), and protein carbonylation (PC) levels in spleen and head kidney were assessed at one, four, seven, and 14 days post-injection. Dose response relationship was explored for data on day four. Phe concentration reached the highest observed level on day four, showed decline on day seven, and was undetectable at the end of trial. EROD activity in both organs followed the pattern of Phe level in all treated groups. Catalase and SOD activity at low Phe concentrations was significantly higher than controls, while LPO and PC level showed no differences from controls. In contrast, at 20 and 40 mg kg-1, CAT and SOD activity, an indicator of oxidative stress, was significantly lower than in untreated controls, while AsA, GSH, LPO, and PC levels were higher on days 4 and 7. No parameter of any treatment group in either organ tissue showed difference from control values at the end of the experiment. The SOD and CAT activity in both organs exhibited a biphasic (initial stimulatory effect) effect, whereas other parameters showed a monophasic effect in terms of dose-response. Results suggest that Phe induced CYP1A and antioxidant responses in immune organs.

• Klíčová slova
• Detoxification, EROD activity, Immune organs, Oxidative damage, Polycyclic aromatic hydrocarbons,
• MeSH
• antioxidancia metabolismus   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• cytochrom P-450 CYP1A1 metabolismus   MeSH
• fenantreny metabolismus   toxicita   MeSH
• glutathion metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• karbonylace proteinů MeSH
• katalasa metabolismus   MeSH
• ledviny metabolismus   MeSH
• mořan zlatý imunologie   metabolismus   fyziologie   MeSH
• oxidace-redukce MeSH
• oxidační stres fyziologie   MeSH
• peroxidace lipidů účinky léků   MeSH
• superoxiddismutasa metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• chemické látky znečišťující vodu MeSH
• cytochrom P-450 CYP1A1 MeSH
• fenantreny MeSH
• glutathion MeSH
• glutathiontransferasa MeSH
• katalasa MeSH
• phenanthrene MeSH Prohlížeč
• superoxiddismutasa MeSH

Semi-volatile organic compounds (SVOCs) are widely distributed across the globe, including polar regions. This study investigates the distribution and bioconcentration of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs) in soils and Colobanthus quitensis, while also estimating potential emission sources. Results indicated high concentrations of PAHs in soils and plants from the Sub-Antarctic region, while OCPs and PCBs were more prevalent in the Antarctic region, with higher contaminant concentrations found in soils than in plant tissues. Hexachlorobenzene (HCB) and dichlorodiphenyldichloroethylene (p,p'-DDE) were significantly higher in the Antarctic region, suggesting historical dichlorodiphenyltrichloroethane (DDT) use, while PCB 153 and 180 were the most representative PCBs in the Antarctic region. Phenanthrene (Phe) was the dominant PAH in both regions. The bioconcentration factor analysis from soils (BCFSoils) revealed potential anthropogenic influences for certain contaminants, including γ-hexachlorocyclohexane (γ-HCH) and PCB 9 in the Sub-Antarctic region, and HCB, p,p'-DDE, PCB 9, and benzo-naphtho-thiophene in the Antarctic region. However, compounds with higher hydrophobicity showed lower Bioconcentration factor (BCFSoils) values, indicating a tendency to accumulate in soil rather than plant tissues. This was consistent with the inverse relationship found between BCFSoils and the octanol-water partition coefficient (Log KOW). Diagnostic ratios of PAHs revealed a predominantly pyrogenic source in the Sub-Antarctic region, while a mixture of sources was observed in the Antarctic region.

• Klíčová slova
• Antarctic, Bioconcentration, Diagnostic ratios, Persistent organic pollutants (POPs) semi-volatile organic compounds (SVOCs), Sub-Antarctic,
• MeSH
• chlorované uhlovodíky analýza   MeSH
• látky znečišťující půdu * analýza   metabolismus   MeSH
• monitorování životního prostředí * MeSH
• pesticidy analýza   metabolismus   MeSH
• polychlorované bifenyly analýza   metabolismus   MeSH
• polycyklické aromatické uhlovodíky analýza   metabolismus   MeSH
• půda chemie   MeSH
• těkavé organické sloučeniny * analýza   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Antarktida MeSH
• Názvy látek
• chlorované uhlovodíky MeSH
• látky znečišťující půdu * MeSH
• pesticidy MeSH
• polychlorované bifenyly MeSH
• polycyklické aromatické uhlovodíky MeSH
• půda MeSH
• těkavé organické sloučeniny * MeSH