A new synthetic method for the fabrication of a sensing layer is presented. PANI films as an ion-to-electron transducer were prepared via acid-assisted polymerization in concentrated formic acid (HCOOH) in the presence of ethanol and ammonium persulfate (APS, as the initiator). The ratio of monomer to ammonium persulfate was 1 : 0.1. 2,2-Bipyridyl, 1,10-phenanthrolin-5-amine, and 8-hydroxyquinoline were used as chelating agents that can complex Fe2+ or Fe3+ ions. The proposed sensors demonstrated an appropriate reproducibility with a rapid response to the presence of Fe2+ or Fe3+ ions, even at T ∼ 37 °C. It was revealed that the method of deposition of a chelating molecule affects the response of sensors. The in situ deposition during acid-assisted polymerization leads to a fast response compared to the layer-by-layer deposition. PMeOx/X1-PANI@FTO and PMeOx/Z1-PANI@FTO sensors exhibit rapid response and are considered a promising detection layer for Fe2+ or Fe3+ ions respectively. We envision that this system can contribute to the next generation of advanced bio-sensors for the potentiometric detection of iron.

• MeSH
• chelátory * MeSH
• ionty MeSH
• polymerizace MeSH
• reprodukovatelnost výsledků MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• ammonium peroxydisulfate MeSH Prohlížeč
• chelátory * MeSH
• ionty MeSH

We constructed a sensor for the determination of Fe2+ and/or Fe3+ ions that consists of a polyaniline layer as an ion-to-electron transducer; on top of it, chelating molecules are deposited (which can selectively chelate specific ions) and protected with a non-biofouling poly(2-methyl-2-oxazoline)s layer. We have shown that our potentiometric sensing layers show a rapid response to the presence of Fe2+ or Fe3+ ions, do not experience interference with other ions (such as Cu2+), and work in a biological environment in the presence of bovine serum albumin (as a model serum protein). The sensing layers detect iron ions in the concentration range from 5 nM to 50 µM.

• Klíčová slova
• analysis, non-biofouling layer, poly(2-methyl-2-oxazoline)s, potentiometry, sensor of Fe2+ or Fe3+ ions,
• MeSH
• aniliny MeSH
• chelátory * MeSH
• elektrody MeSH
• iontově selektivní elektrody * MeSH
• ionty MeSH
• koncentrace vodíkových iontů MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aniliny MeSH
• chelátory * MeSH
• ionty MeSH
• polyaniline MeSH Prohlížeč