-
Je něco špatně v tomto záznamu ?
Comparative proteome analysis of rat brain and coronary microvascular endothelial cells
L. Lu, P. Y. Yang, Y. Ch. Rui, H. Kang, J. Zhang, J. P. Zhang, W. H. Feng
Jazyk angličtina Země Česko
NLK
Directory of Open Access Journals
od 1991
Free Medical Journals
od 1998
ProQuest Central
od 2005-01-01
Medline Complete (EBSCOhost)
od 2006-01-01
Nursing & Allied Health Database (ProQuest)
od 2005-01-01
Health & Medicine (ProQuest)
od 2005-01-01
ROAD: Directory of Open Access Scholarly Resources
od 1998
- MeSH
- adaptorové proteiny signální transdukční analýza chemie MeSH
- cévní endotel cytologie patofyziologie patologie MeSH
- finanční podpora výzkumu jako téma MeSH
- kardiovaskulární fyziologické jevy MeSH
- mikrocirkulace genetika fyziologie patofyziologie MeSH
- mozkový krevní oběh fyziologie MeSH
- potkani Wistar anatomie a histologie MeSH
- proteiny analýza fyziologie chemie MeSH
- spektrální analýza metody využití MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
The endothelium of different organs displays a remarkable heterogeneity, although it presents many common functional and morphological features. However, despite our knowledge of heterogeneity among endothelial cells from different sites, the differences between brain microvascular endothelial cells (BMEC) and coronary microvascular endothelial cells (CMEC) are poorly defined. The aim of this study was to investigate whether BMEC are distinct from CMEC at the protein level. Using the proteomic approach, we comparatively analyzed the proteome of cultured BMEC and CMEC. We reproducibly separated over 2000 polypeptides by using two-dimensional electrophoresis (2-DE) at pH range of 3-10. Using PDQuest software to process the 2-DE gel images, forty-seven protein spots were differentially expressed in the two-endothelial cells. Of these, thirty-five proteins are highly expressed in BMEC, whereas twelve proteins are highly expressed in CMEC. Fifteen proteins in BMEC and seven proteins in CMEC were identified with high confidence by matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). Our data suggested that BMEC and CMEC were different in several aspects including cytokine and growth-related molecules, stress-related proteins, metabolic enzymes, signal transduction proteins and others. The identification of a set of proteins preferentially expressed in BMEC and CMEC provided new data on the heterogeneity of the endothelium.
Grant č. 30271509 -- Grant č. 39870870 -- Grant č. 30300454 -- Grant č. 2002AA2Z346C
Bibliografie atd.Lit.: 33
- 000
- 03638naa 2200493 a 4500
- 001
- bmc07502292
- 003
- CZ-PrNML
- 005
- 20111210121407.0
- 008
- 080304s2007 xr e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Lu, L.
- 245 10
- $a Comparative proteome analysis of rat brain and coronary microvascular endothelial cells / $c L. Lu, P. Y. Yang, Y. Ch. Rui, H. Kang, J. Zhang, J. P. Zhang, W. H. Feng
- 314 __
- $a Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai
- 500 __
- $a Grant č. 30271509 -- Grant č. 39870870 -- Grant č. 30300454 -- Grant č. 2002AA2Z346C
- 504 __
- $a Lit.: 33
- 520 9_
- $a The endothelium of different organs displays a remarkable heterogeneity, although it presents many common functional and morphological features. However, despite our knowledge of heterogeneity among endothelial cells from different sites, the differences between brain microvascular endothelial cells (BMEC) and coronary microvascular endothelial cells (CMEC) are poorly defined. The aim of this study was to investigate whether BMEC are distinct from CMEC at the protein level. Using the proteomic approach, we comparatively analyzed the proteome of cultured BMEC and CMEC. We reproducibly separated over 2000 polypeptides by using two-dimensional electrophoresis (2-DE) at pH range of 3-10. Using PDQuest software to process the 2-DE gel images, forty-seven protein spots were differentially expressed in the two-endothelial cells. Of these, thirty-five proteins are highly expressed in BMEC, whereas twelve proteins are highly expressed in CMEC. Fifteen proteins in BMEC and seven proteins in CMEC were identified with high confidence by matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). Our data suggested that BMEC and CMEC were different in several aspects including cytokine and growth-related molecules, stress-related proteins, metabolic enzymes, signal transduction proteins and others. The identification of a set of proteins preferentially expressed in BMEC and CMEC provided new data on the heterogeneity of the endothelium.
- 650 _2
- $a cévní endotel $x cytologie $x patofyziologie $x patologie $7 D004730
- 650 _2
- $a mozkový krevní oběh $x fyziologie $7 D002560
- 650 _2
- $a kardiovaskulární fyziologické jevy $7 D002320
- 650 _2
- $a proteiny $x analýza $x fyziologie $x chemie $7 D011506
- 650 _2
- $a potkani Wistar $x anatomie a histologie $7 D017208
- 650 _2
- $a mikrocirkulace $x genetika $x fyziologie $x patofyziologie $7 D008833
- 650 _2
- $a spektrální analýza $x metody $x využití $7 D013057
- 650 _2
- $a adaptorové proteiny signální transdukční $x analýza $x chemie $7 D048868
- 650 _2
- $a finanční podpora výzkumu jako téma $7 D012109
- 650 _2
- $a zvířata $7 D000818
- 700 1_
- $a Yang, P. Y.
- 700 1_
- $a Rui, Y. Ch.
- 700 1_
- $a Kang, H.
- 700 1_
- $a Zhang, J.
- 700 1_
- $a Zhang, J. P.
- 700 1_
- $a Feng, W. H.
- 773 0_
- $w MED00003824 $t Physiological research $g Roč. 56, č. 2 (2007), s. 159-168 $x 0862-8408
- 856 41
- $u http://www.biomed.cas.cz/physiolres/pdf/56/56_159.pdf $y plný text volně přístupný
- 910 __
- $a ABA008 $b A 4120 $c 266 $y 1
- 990 __
- $a 20080304105411 $b ABA008
- 991 __
- $a 20080305161720 $b ABA008
- 999 __
- $a ok $b bmc $g 617912 $s 470344
- BAS __
- $a 3
- BMC __
- $a 2007 $b 56 $c 2 $d 159-168 $i 0862-8408 $m Physiological research $x MED00003824
- LZP __
- $a 2007-22/mkal