Immunosuppressive protocol with delayed use of low-dose tacrolimus after aortic transplantation suppresses donor-specific anti-MHC class I and class II antibody production in rats
Jazyk angličtina Země Spojené státy americké Médium electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24815872
DOI
10.12659/aot.889870
PII: 889870
Knihovny.cz E-zdroje
- MeSH
- aorta transplantace MeSH
- B-lymfocyty imunologie MeSH
- CD8-pozitivní T-lymfocyty imunologie MeSH
- histokompatibilita - antigeny třídy I imunologie MeSH
- histokompatibilita - antigeny třídy II imunologie MeSH
- homologní transplantace MeSH
- imunosupresiva farmakologie MeSH
- isoprotilátky imunologie MeSH
- krysa rodu Rattus MeSH
- potkani inbrední BN MeSH
- potkani inbrední LEW MeSH
- rejekce štěpu farmakoterapie imunologie MeSH
- slezina cytologie imunologie MeSH
- takrolimus farmakologie MeSH
- transplantace cév metody MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- histokompatibilita - antigeny třídy I MeSH
- histokompatibilita - antigeny třídy II MeSH
- imunosupresiva MeSH
- isoprotilátky MeSH
- takrolimus MeSH
BACKGROUND: Arterial allografts are used as vascular conduits in the treatment of prosthetic graft infection. Immunosuppression decreases their rupture risk rate. However, immunosuppression can be unprofitable in florid infection. Previously, we confirmed inhibition of cell-mediated destruction of rat aortic grafts by delayed use of tacrolimus. In this work, we studied the influence of this protocol on the antibody-mediated rejection. MATERIAL AND METHODS: Flow cytometry was used for the retrospective analysis of day 0, 14, and 30 sera obtained from Lewis rat recipients of isogeneic fresh infrarenal aortic grafts (group A) or Brown-Norway rat aortic grafts (group B,C,D) for the presence of donor-specific anti-MHC class I and II antibodies. Tacrolimus in daily dose of 0.2 mg/kg was administered from day 1 to day 30 (group C) or from day 7 to day 30 (group D). RESULTS: Inhibition of fluorescence-labeled anti-BN MHC class I and MHC class II antibodies binding to BN-splenocytes was observed only by day 14 and day 30 sera of allogeneic non-immunosuppressed Lewis rats (group B). The day 30 sera significantly decreased anti-MHC I (42±3%) and anti-MHC II antibody binding (56±3%) compared to day 0 (76±9%, p=0.005 and 79±5%, p=0.003, respectively). Deposition of immunoglobulins G into the tunica media was observed only in non-immunosuppressed aortic allografts on day 30. CONCLUSIONS: Fresh aortic allografts induce donor-specific anti-MHC class I and anti-MHC class II antibody production. Delayed administration of tacrolimus completely suppressed antibody production and antibody-mediated destruction of aortic allografts.
Citace poskytuje Crossref.org
Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats
