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Analysis of the molecular variability of Grapevine leafroll-associated virus 1 reveals the presence of two distinct virus groups and their mixed occurrence in grapevines
Komínek P, Glasa M, Bryxiová M.
Jazyk angličtina Země Spojené státy americké
NLK
ProQuest Central
od 1997-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-01-01 do Před 1 rokem
Public Health Database (ProQuest)
od 1997-01-01 do Před 1 rokem
- MeSH
- Closteroviridae genetika izolace a purifikace patogenita MeSH
- DNA virů genetika MeSH
- financování organizované MeSH
- fylogeneze MeSH
- genetická variace MeSH
- klonování DNA MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin virologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- proteiny tepelného šoku HSP70 genetika MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie aminokyselin MeSH
- virové geny MeSH
- virové proteiny genetika MeSH
- Vitis virologie MeSH
- Geografické názvy
- Česká republika MeSH
- Slovenská republika MeSH
Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.
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- $a Analysis of the molecular variability of Grapevine leafroll-associated virus 1 reveals the presence of two distinct virus groups and their mixed occurrence in grapevines / $c Komínek P, Glasa M, Bryxiová M.
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- $a Research Institute of Crop Production, Drnovská 507, 161 06, Prague 6, Ruzyne, Czech Republic. kominek@vurv.cz
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- $a Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.
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