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Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex
Lenka Fišerová, Kateřina Černá, Helena Horká, Jan Kopecký
Jazyk angličtina Země Česko
NLK
Free Medical Journals
od 1966
ProQuest Central
od 2004-01-01 do Před 3 měsíci
Health & Medicine (ProQuest)
od 2004-01-01 do Před 3 měsíci
Public Health Database (ProQuest)
od 2004-01-01 do Před 3 měsíci
ROAD: Directory of Open Access Scholarly Resources
od 1982
- MeSH
- Borrelia burgdorferi komplex růst a vývoj MeSH
- DNA bakterií genetika MeSH
- ELISA MeSH
- klíště mikrobiologie MeSH
- larva mikrobiologie MeSH
- lymeská nemoc mikrobiologie přenos MeSH
- myši MeSH
- nymfa mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- protilátky bakteriální krev MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
A previously reported procedure for the introduction of Borrelia spirochetes into tick larvae by immersion in a suspension of spirochetes was tested on Ixodes ricinus (L.) ticks and three of the most medically important European Borrelia genomic species, B. burgdorferi sensu stricto, B. garinii and B. afzelii. The procedure was compared with “classical” infection of nymphs by feeding on infected mice. Both methods yielded comparable results (infection rate 44–65%) with the exception of B. afzelii, which produced better results using the immersion method (44%) compared with feeding on infected mice (16%). Nymphs infected by the immersion method at the larval stage were able to transmit the infection to naive mice as shown by serology and PCR detection of spirochetal DNA in organs. The immersion method is faster than feeding on infected mice and provides more reproducible conditions for infection. It can be exploited for studies on both pathogen transmission and Borrelia-vector interactions.
Citace poskytuje Crossref.org
Lit.: 19
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- $a A previously reported procedure for the introduction of Borrelia spirochetes into tick larvae by immersion in a suspension of spirochetes was tested on Ixodes ricinus (L.) ticks and three of the most medically important European Borrelia genomic species, B. burgdorferi sensu stricto, B. garinii and B. afzelii. The procedure was compared with “classical” infection of nymphs by feeding on infected mice. Both methods yielded comparable results (infection rate 44–65%) with the exception of B. afzelii, which produced better results using the immersion method (44%) compared with feeding on infected mice (16%). Nymphs infected by the immersion method at the larval stage were able to transmit the infection to naive mice as shown by serology and PCR detection of spirochetal DNA in organs. The immersion method is faster than feeding on infected mice and provides more reproducible conditions for infection. It can be exploited for studies on both pathogen transmission and Borrelia-vector interactions.
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