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Chitinolytic activity of the sheep rumen ciliate Diploplastron affine
G.Bełzecki, R. Miltko, T. Michałowski, Jiří Šimůnek, Jan Kopečný
Jazyk angličtina Země Česko
- MeSH
- bachor metabolismus parazitologie MeSH
- chitin metabolismus MeSH
- chitinasy chemie klasifikace metabolismus MeSH
- Ciliophora enzymologie růst a vývoj MeSH
- kultivační média chemie MeSH
- ovce metabolismus parazitologie MeSH
- protozoální proteiny klasifikace metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Supplementation of the rumen ciliate Diploplastron affine growth medium with commercial chitin stimulated growth of ciliates and the density of their population was positively correlated with chitin doses (r = 0.95; p < 0.01). The cell-free extracts prepared from bacteria-free ciliates degraded chitin to N-acetyl-D: -glucosamine and chitobiose. Three exochitinases, two endochitinases and two beta-N-acetylglucosaminidases were identified in the cell-free extract of protozoa. The molar mass of exochitinases was 80, 65 and 30 kDa, and endochitinases 75 and 50 kDa; the molar mass of one of the identified beta-N-acetylglucosaminidases was 45 kDa.
Lit.: 16
- 000
- 00000naa 2200000 a 4500
- 001
- bmc07513586
- 003
- CZ-PrNML
- 005
- 20111210124650.0
- 008
- 081210s2008 xr e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
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- $a xr
- 100 1_
- $a Bełzecki, G.
- 245 10
- $a Chitinolytic activity of the sheep rumen ciliate Diploplastron affine / $c G.Bełzecki, R. Miltko, T. Michałowski, Jiří Šimůnek, Jan Kopečný
- 314 __
- $a The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna
- 504 __
- $a Lit.: 16
- 520 9_
- $a Supplementation of the rumen ciliate Diploplastron affine growth medium with commercial chitin stimulated growth of ciliates and the density of their population was positively correlated with chitin doses (r = 0.95; p < 0.01). The cell-free extracts prepared from bacteria-free ciliates degraded chitin to N-acetyl-D: -glucosamine and chitobiose. Three exochitinases, two endochitinases and two beta-N-acetylglucosaminidases were identified in the cell-free extract of protozoa. The molar mass of exochitinases was 80, 65 and 30 kDa, and endochitinases 75 and 50 kDa; the molar mass of one of the identified beta-N-acetylglucosaminidases was 45 kDa.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a chitin $x metabolismus $7 D002686
- 650 _2
- $a chitinasy $x chemie $x klasifikace $x metabolismus $7 D002688
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- $a Ciliophora $x enzymologie $x růst a vývoj $7 D016798
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- $a protozoální proteiny $x klasifikace $x metabolismus $7 D015800
- 650 _2
- $a bachor $x metabolismus $x parazitologie $7 D012417
- 650 _2
- $a ovce $x metabolismus $x parazitologie $7 D012756
- 700 1_
- $a Miltko, R.
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- $a Michałowski, T.
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- $a Šimůnek, Jiří $7 xx0106321
- 700 1_
- $a Kopečný, Jan $7 xx0080357
- 773 0_
- $w MED00011005 $t Folia microbiologica $g Roč. 53, č. 3 (2008), s. 201-203 $x 0015-5632
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- $a ok $b bmc $g 629181 $s 481634
- BAS __
- $a 3
- BMC __
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- LZP __
- $a 2008-22/mkme
