The diversity of archaebacteria associated with anaerobic rumen protozoan Entodinium caudatum in long term in vitro culture was investigated by denaturing gradient gel electrophoresis (DGGE) analysis of hypervariable V3 region of archaebacterial 16S rRNA gene. PCR was accomplished directly from DNA extracted from a single protozoal cell and from total community genomic DNA and the obtained fingerprints were compared. The analysis indicated the presence of a solitary intensive band present in Entodinium caudatum single cell DNA, which had no counterparts in the profile from total DNA. The identity of archaebacterium represented by this band was determined by sequence analysis which showed that the sequence fell to the cluster of ciliate symbiotic methanogens identified recently by 16S gene library approach.
- MeSH
- anaerobióza MeSH
- Archaea genetika izolace a purifikace klasifikace růst a vývoj MeSH
- bachor mikrobiologie parazitologie MeSH
- Ciliophora genetika izolace a purifikace růst a vývoj MeSH
- DNA archebakterií analýza izolace a purifikace MeSH
- druhová specificita MeSH
- fylogeneze MeSH
- kultivační média MeSH
- molekulární sekvence - údaje MeSH
- ovce MeSH
- protozoální DNA analýza izolace a purifikace MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- symbióza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Supplementation of the rumen ciliate Diploplastron affine growth medium with commercial chitin stimulated growth of ciliates and the density of their population was positively correlated with chitin doses (r = 0.95; p < 0.01). The cell-free extracts prepared from bacteria-free ciliates degraded chitin to N-acetyl-D: -glucosamine and chitobiose. Three exochitinases, two endochitinases and two beta-N-acetylglucosaminidases were identified in the cell-free extract of protozoa. The molar mass of exochitinases was 80, 65 and 30 kDa, and endochitinases 75 and 50 kDa; the molar mass of one of the identified beta-N-acetylglucosaminidases was 45 kDa.
- MeSH
- bachor metabolismus parazitologie MeSH
- chitin metabolismus MeSH
- chitinasy chemie klasifikace metabolismus MeSH
- Ciliophora enzymologie růst a vývoj MeSH
- kultivační média chemie MeSH
- ovce metabolismus parazitologie MeSH
- protozoální proteiny klasifikace metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- Ciliophora patogenita růst a vývoj ultrastruktura MeSH
- finanční podpora výzkumu jako téma MeSH
- infekce prvoky kmene Ciliophora prevence a kontrola MeSH
- kultivační média MeSH
- LD50 MeSH
- ryby parazitologie MeSH
- techniky in vitro MeSH
- teplota MeSH
- virulence MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
