-
Something wrong with this record ?
Partial characterization of Enterococcus faecalis bacteriophage F4
Katarína Nigutová, Igor Štyriak, Peter Javorský, Peter Pristaš
Language English Country Czech Republic
- MeSH
- Bacteriophages physiology genetics classification MeSH
- Enterococcus faecalis virology MeSH
- Genome, Viral MeSH
- Lysogeny MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Sequence Analysis, DNA MeSH
- Viral Proteins genetics MeSH
Large Enterococcus faecalis F4 bacteriophage (described earlier) consisting of double-stranded linear DNA of approximately 60 kb was characterized. Library was prepared of its random DNA fragments and selected recombinants were sequenced. Three phage essential genes were characterized: DNA polymerase, replicative DNA helicase and a minor capsid protein, showing only limited homology to other known phage encoded genes. The occurrence of these genes among enterococci was determined by PCR method. Only two out of 40 tested isolates possessed all three genes, another three isolates contained at least one of the genes, demonstrating low frequency F4 lysogens among natural enterococcal isolates.
Lit.: 9
- 000
- 00000naa 2200000 a 4500
- 001
- bmc07513662
- 003
- CZ-PrNML
- 005
- 20111210124703.0
- 008
- 081209s2008 xr e eng||
- 009
- AR
- 024 7_
- $2 doi $a 10.1007/s12223-008-0033-y
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Nigutová, Katarína. $7 _AN024011
- 245 10
- $a Partial characterization of Enterococcus faecalis bacteriophage F4 / $c Katarína Nigutová, Igor Štyriak, Peter Javorský, Peter Pristaš
- 314 __
- $a Institute of Animal Physiology, Slovak Academy of Sciences, Košice
- 504 __
- $a Lit.: 9
- 520 9_
- $a Large Enterococcus faecalis F4 bacteriophage (described earlier) consisting of double-stranded linear DNA of approximately 60 kb was characterized. Library was prepared of its random DNA fragments and selected recombinants were sequenced. Three phage essential genes were characterized: DNA polymerase, replicative DNA helicase and a minor capsid protein, showing only limited homology to other known phage encoded genes. The occurrence of these genes among enterococci was determined by PCR method. Only two out of 40 tested isolates possessed all three genes, another three isolates contained at least one of the genes, demonstrating low frequency F4 lysogens among natural enterococcal isolates.
- 650 _2
- $a bakteriofágy $x fyziologie $x genetika $x klasifikace $7 D001435
- 650 _2
- $a Enterococcus faecalis $x virologie $7 D013293
- 650 _2
- $a genom virový $7 D016679
- 650 _2
- $a lyzogenie $7 D008242
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a polymerázová řetězová reakce $7 D016133
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 650 _2
- $a virové proteiny $x genetika $7 D014764
- 700 1_
- $a Štyriak, Igor. $7 xx0248774
- 700 1_
- $a Javorský, Peter, $d 1950- $7 xx0072696
- 700 1_
- $a Pristaš, Peter $7 xx0101869
- 773 0_
- $w MED00011005 $t Folia microbiologica $g Roč. 53, č. 3 (2008), s. 234-236 $x 0015-5632
- 910 __
- $a ABA008 $b A 2403 $c 561 $y 1
- 990 __
- $a 20081205115046 $b ABA008
- 991 __
- $a 20090511155023 $b ABA008
- 999 __
- $a ok $b bmc $g 629257 $s 481710
- BAS __
- $a 3
- BMC __
- $a 2008 $b 53 $c 3 $d 234-236 $i 0015-5632 $m Folia microbiologica $x MED00011005
- LZP __
- $a 2008-22/mkme
