-
Something wrong with this record ?
A new method of optical detection of yeast acidification power
Petr Gabriel, Miroslav Dienstbier, Petr Sladký, Karel Sigler
Language English Country Czech Republic
- MeSH
- Bromcresol Green analysis MeSH
- Fermentation MeSH
- Glucose metabolism MeSH
- Indicators and Reagents analysis MeSH
- Colorimetry methods instrumentation MeSH
- Hydrogen-Ion Concentration MeSH
- Culture Media, Conditioned chemistry MeSH
- Yeasts metabolism MeSH
- Mycology methods instrumentation MeSH
- Nephelometry and Turbidimetry methods instrumentation MeSH
We describe here a newly developed method for a contact-free optical pH measurement in yeast suspensions supplemented with glucose, and containing the pH sensitive triphenylmethane dye bromocresol green. It is suitable for performing the acidification power test (based on measuring the rate of pH drop of yeast suspension caused by active extrusion of acidity from cells after glucose addition) used for assessing yeast vitality in fermentation industries. Using this methodology we monitored the pH in yeast suspensions in the course of acidification in the pH range of 3.5-5.3. Optical pH measurement allows simultaneous testing of several samples, minimizes the sample volume, simplifies sample handling and reduces the hands-on time in sample processing.
Lit.: 9
- 000
- 00000naa 2200000 a 4500
- 001
- bmc07523531
- 003
- CZ-PrNML
- 005
- 20111210135727.0
- 008
- 090519s2008 xr e eng||
- 009
- AR
- 024 7_
- $2 doi $a 10.1007/s12223-008-0083-1
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Gabriel, Petr $7 xx0108103
- 245 12
- $a A new method of optical detection of yeast acidification power / $c Petr Gabriel, Miroslav Dienstbier, Petr Sladký, Karel Sigler
- 314 __
- $a Faculty yof Mathematics and Physics, Charles University, Prague
- 504 __
- $a Lit.: 9
- 520 9_
- $a We describe here a newly developed method for a contact-free optical pH measurement in yeast suspensions supplemented with glucose, and containing the pH sensitive triphenylmethane dye bromocresol green. It is suitable for performing the acidification power test (based on measuring the rate of pH drop of yeast suspension caused by active extrusion of acidity from cells after glucose addition) used for assessing yeast vitality in fermentation industries. Using this methodology we monitored the pH in yeast suspensions in the course of acidification in the pH range of 3.5-5.3. Optical pH measurement allows simultaneous testing of several samples, minimizes the sample volume, simplifies sample handling and reduces the hands-on time in sample processing.
- 650 _2
- $a bromkresolová zeleň $x analýza $7 D001961
- 650 _2
- $a kolorimetrie $x metody $x přístrojové vybavení $7 D003124
- 650 _2
- $a kultivační média speciální $x chemie $7 D017077
- 650 _2
- $a fermentace $7 D005285
- 650 _2
- $a glukosa $x metabolismus $7 D005947
- 650 _2
- $a koncentrace vodíkových iontů $7 D006863
- 650 _2
- $a indikátory a reagencie $x analýza $7 D007202
- 650 _2
- $a mykologie $x metody $x přístrojové vybavení $7 D009172
- 650 _2
- $a nefelometrie a turbidimetrie $x metody $x přístrojové vybavení $7 D009391
- 650 _2
- $a kvasinky $x metabolismus $7 D015003
- 700 1_
- $a Dienstbier, Miroslav $7 xx0097033
- 700 1_
- $a Sladký, Petr $7 xx0097034
- 700 1_
- $a Sigler, Karel, $d 1942- $7 xx0102701
- 773 0_
- $w MED00011005 $t Folia microbiologica $g Roč. 53, č. 6 (2008), s. 527-533 $x 0015-5632
- 910 __
- $a ABA008 $b A 2403 $c 561 $y 9
- 990 __
- $a 20090519101452 $b ABA008
- 991 __
- $a 20090521080943 $b ABA008
- 999 __
- $a ok $b bmc $g 655569 $s 508879
- BAS __
- $a 3
- BMC __
- $a 2008 $b 53 $c 6 $d 527-533 $i 0015-5632 $m Folia microbiologica $x MED00011005
- LZP __
- $a 2009-18/mkme
