Background: Midazolam is a frequently used benzodiazepine in anaesthesiology and intensive care.Aim: The aim of pilot study was to monitor its eff ect during heart perfusion in the laboratory rat.Methods: The same groups of animals (n = 10). The 1st group was treated with midazolam in a dose of 0.5mg/kgi.p. The 2nd group was a placebo. After i.p. administration of heparine injection of 500 IU dose, the hearts were excisedand perfused (modifi ed Langendorf’s method). Working schedule: stabilization/ischaemia/reperfusion proceed at intervalsof 20/30/60 min. Monitored parameters in isolated heart: left ventricle pressure (LVP), end-diastolic pressure(LVEDP), contractility (+dP/dtmax).Results: The treated hearts showed improved postischemic recovery, reaching LVP values of 92 ± 6 % at the end ofthe reperfusion, placebo only 61 ± 7 %. In placebo hearts LVEDP rose from 10.0 ± 0.5 mmHg to 43 ± 4 mmHg after,in treated animals only about 25 mmHg. The treated hearts improved +dP/dtmax recovery during reperfusion to 91 ±8 %. These values were signifi cantly greater than those obtained from the placebo hearts.Conclusions: Positive changes in monitored parameters were found in this experimental pilot study. We concludethat the administration of midazolam in laboratory rats has a cardioprotective potential against ischemia-reperfusioninduced injury.

Department of Physiology Faculty of Medicine and Dentistry Palacky University Olomouc

References provided by Crossref.org

Lit.: 39