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PCR-DGGE analysis of bacterial population attached to the bovine rumen wall
F. Lukáš, J. Šimůnek, J. Mrázek, J. Kopečný
Folia microbiologica.
2010 ;
55 (4) :
345-348.
Jazyk angličtina Země Česko
Perzistentní odkaz
https://www.medvik.cz/link/bmc10025448
- MeSH
- bachor mikrobiologie MeSH
- Bacteria genetika klasifikace MeSH
- biodiverzita MeSH
- denaturace nukleových kyselin MeSH
- DNA bakterií genetika MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- financování organizované MeSH
- polymerázová řetězová reakce MeSH
- ribozomální DNA genetika MeSH
- RNA ribozomální 16S genetika MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
We isolated and amplified by PCR 16S rDNA from bacteria attached to the bovine rumen wall and analyzed it by denaturing gradient gel electrophoresis (DGGE) with subsequent sequence analysis. The attached bacterial community differed from the bacteria of rumen content; however, no differences were observed among the five epithelial sampling sites taken from each animal. The DGGE profile of the bacterial population attached to the rumen wall represented a high inter-animal variation.
6th International Symposium of Anaerobic Microbiology (ISAM 2009) held in Liblice (Czech Republic), 18 - 20 June 2009
Bibliografie atd.Lit.: 15
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