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Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus
D Ruzek, TS Gritsun, NL Forrester, EA Gould, J Kopecky, M Golovchenko, N Rudenko, L Grubhoffer
Jazyk angličtina Země Spojené státy americké
Perzistentní odkaz
https://www.medvik.cz/link/bmc11003255
- MeSH
- financování organizované MeSH
- genetika MeSH
- klíšťata virologie MeSH
- klíšťová encefalitida mortalita patofyziologie virologie MeSH
- kojená zvířata MeSH
- molekulární modely MeSH
- mozek virologie MeSH
- mutace MeSH
- myši inbrední ICR MeSH
- myši MeSH
- RNA-helikasy genetika chemie MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- selekce (genetika) MeSH
- serinové endopeptidasy genetika chemie MeSH
- sériové pasážování MeSH
- substituce aminokyselin MeSH
- virové nestrukturální proteiny genetika chemie MeSH
- virulence MeSH
- viry klíšťové encefalitidy genetika izolace a purifikace patogenita MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Geografické názvy
- Evropa MeSH
An attenuated strain (263) of the tick-borne encephalitis virus, isolated from field ticks, was either serially subcultured, 5 times in mice, or at 40 degrees C in PS cells, producing 2 independent strains, 263-m5 and 263-TR with identical genomes; both strains exhibited increased plaque size, neuroinvasiveness and temperature-resistance. Sequencing revealed two unique amino acid substitutions, one mapping close to the catalytic site of the viral protease. These observations imply that virus adaptation from ticks to mammals occurs by selection of pre-existing virulent variants from the quasispecies population rather than by the emergence of new random mutations. The significance of these observations is discussed.
Citace poskytuje Crossref.org
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