Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I

E Sisakova, LK Stanley, M Weiserova, MD Szczelkun

. 2008 ; 36 (12) : 3939-3949.

Language English Country Great Britain

Persistent link   https://www.medvik.cz/link/bmc11004018

The Type I restriction-modification enzyme EcoR124I is an ATP-dependent endonuclease that uses dsDNA translocation to locate and cleave distant non-specific DNA sites. Bioinformatic analysis of the HsdR subunits of EcoR124I and related Type I enzymes showed that in addition to the principal PD-(E/D)xK Motifs, I, II and III, a QxxxY motif is also present that is characteristic of RecB-family nucleases. The QxxxY motif resides immediately C-terminal to Motif III within a region of predicted alpha-helix. Using mutagenesis, we examined the role of the Q and Y residues in DNA binding, translocation and cleavage. Roles for the QxxxY motif in coordinating the catalytic residues or in stabilizing the nuclease domain on the DNA are discussed.

References provided by Crossref.org

000      
02323naa 2200409 a 4500
001      
bmc11004018
003      
CZ-PrNML
005      
20111210202519.0
008      
110303s2008 xxk e eng||
009      
AR
024    __
$a 10.1093/nar/gkn333 $2 doi
035    __
$a (PubMed)18511464
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Šišáková, Eva. $7 xx0222544
245    12
$a A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I / $c E Sisakova, LK Stanley, M Weiserova, MD Szczelkun
314    __
$a Institute of Microbiology v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic.
520    9_
$a The Type I restriction-modification enzyme EcoR124I is an ATP-dependent endonuclease that uses dsDNA translocation to locate and cleave distant non-specific DNA sites. Bioinformatic analysis of the HsdR subunits of EcoR124I and related Type I enzymes showed that in addition to the principal PD-(E/D)xK Motifs, I, II and III, a QxxxY motif is also present that is characteristic of RecB-family nucleases. The QxxxY motif resides immediately C-terminal to Motif III within a region of predicted alpha-helix. Using mutagenesis, we examined the role of the Q and Y residues in DNA binding, translocation and cleavage. Roles for the QxxxY motif in coordinating the catalytic residues or in stabilizing the nuclease domain on the DNA are discussed.
650    _2
$a aminokyselinové motivy $7 D020816
650    _2
$a sekvence aminokyselin $7 D000595
650    _2
$a substituce aminokyselin $7 D019943
650    _2
$a DNA $x metabolismus $7 D004247
650    _2
$a restrikční endonukleasy typu I $x genetika $x chemie $x metabolismus $7 D015253
650    _2
$a exodeoxyribonukleasa V $x chemie $7 D043211
650    _2
$a kinetika $7 D007700
650    _2
$a molekulární sekvence - údaje $7 D008969
650    _2
$a mutageneze $7 D016296
650    _2
$a podjednotky proteinů $x chemie $7 D021122
650    _2
$a transport proteinů $7 D021381
650    _2
$a sekvenční seřazení $7 D016415
650    _2
$a financování organizované $7 D005381
700    1_
$a Stanley, Louise K.
700    1_
$a Weiserová, Marie. $7 _AN043251
700    1_
$a Szczelkun, Mark D.
773    0_
$t Nucleic Acids Research $w MED00003554 $g Roč. 36, č. 12 (2008), s. 3939-3949
910    __
$a ABA008 $b x $y 1
990    __
$a 20110413122738 $b ABA008
991    __
$a 20110413122738 $b ABA008
999    __
$a ok $b bmc $g 831358 $s 696043
BAS    __
$a 3
BMC    __
$a 2008 $b 36 $c 12 $d 3939-3949 $m Nucleic acids research $n Nucleic Acids Res $x MED00003554
LZP    __
$a 2011-3B/ipme

Find record

Citation metrics