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Pseudomonas aeruginosa PAO1 adapted to 2-phenoxyethanol shows cross-resistance to dissimilar biocides and increased susceptibility to antibiotics
S.M.A. Abdel Malek, Y.R. Badran
Language English Country Czech Republic
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Adaptation, Biological MeSH
- Disinfectants metabolism toxicity MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Ethylene Glycols metabolism toxicity MeSH
- Financing, Organized MeSH
- Microbial Sensitivity Tests MeSH
- Bacterial Outer Membrane Proteins analysis MeSH
- Proteome analysis MeSH
- Pseudomonas aeruginosa physiology MeSH
- Serial Passage MeSH
- Drug Tolerance MeSH
The growth adaptability to increasing concentration of the biocide 2-phenoxyethanol (PE) was determined in Pseudomonas aeruginosa PAO1 (P.a.) as part of efforts to understand and control the biocide tolerance and its effect on cross-resistance to other biocides and resistance to antibiotics. After repeated subculturing in media containing increasing sub-minimum-inhibitory PE concentration, P.a. exhibited an adaptive resistance indicated by two-fold increase in MIC at the 10th passage. The resistance was stable and remained after passaging the strain in further 7 successive passages in PE-free growth media. The strain showed cross-resistance towards dissimilar biocides and displayed increased susceptibility to antibiotics, which was not influenced by the presence of the efflux inhibitor 'carbonyl cyanide m-chlorophenyl hydrazone'. Outer membranes of adapted strain showed altered protein profile when examined by SDS-PAGE.
Obsahuje 4 tabulky
Bibliography, etc.Literatura
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- $2 doi $a 10.1007/s12223-010-0094-6
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- $a Abdel Malek, S. M. A. $u Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Biomedical Sciences, Petra University, Amman, Jordan $7 gn_A_00000292
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- $a Pseudomonas aeruginosa PAO1 adapted to 2-phenoxyethanol shows cross-resistance to dissimilar biocides and increased susceptibility to antibiotics / $c S.M.A. Abdel Malek, Y.R. Badran
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- $a Obsahuje 4 tabulky
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- $a Literatura
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- $a The growth adaptability to increasing concentration of the biocide 2-phenoxyethanol (PE) was determined in Pseudomonas aeruginosa PAO1 (P.a.) as part of efforts to understand and control the biocide tolerance and its effect on cross-resistance to other biocides and resistance to antibiotics. After repeated subculturing in media containing increasing sub-minimum-inhibitory PE concentration, P.a. exhibited an adaptive resistance indicated by two-fold increase in MIC at the 10th passage. The resistance was stable and remained after passaging the strain in further 7 successive passages in PE-free growth media. The strain showed cross-resistance towards dissimilar biocides and displayed increased susceptibility to antibiotics, which was not influenced by the presence of the efflux inhibitor 'carbonyl cyanide m-chlorophenyl hydrazone'. Outer membranes of adapted strain showed altered protein profile when examined by SDS-PAGE.
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- $a Badran, Y. R. $u Faculty of Medicine, Jordan University, Amman, Jordan
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- $t Folia microbiologica $x 0015-5632 $g Roč. 55, č. 6 (2010), s. 588-592 $w MED00011005
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