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New haemolytic potential assay on an alternative insect model
J Berger
Jazyk angličtina Země Dánsko
Typ dokumentu práce podpořená grantem
NLK
Medline Complete (EBSCOhost)
od 2004-01-01 do Před 1 rokem
Wiley Online Library (archiv)
od 1997-01-01 do 2012-12-31
- MeSH
- alternativy testů na zvířatech MeSH
- fenylhydraziny toxicita MeSH
- hemocyty cytologie účinky léků MeSH
- hemolýza MeSH
- larva cytologie MeSH
- modely u zvířat MeSH
- počet buněk MeSH
- Spodoptera cytologie MeSH
- testy toxicity metody MeSH
- xenobiotika toxicita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
A new screening test of a haemolytic potential is presented on Spodoptera larvae in vivo. The assay was evaluated using phenylhydrazine, the well-known xenobiotic which induces haemolysis in mammals. Haemocyte disintegration causes changes in total haemocyte counts and differentials. It is shown that only qualitative evaluation of panoptically stained haemolymph smears is sufficient, fast and cheap to detect haemolytic potential. The presented assay also prevents unneeded, non-ethical use of vertebrates.
Citace poskytuje Crossref.org
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- $a Berger, Josef, $d 1949- $7 jn19990010050 $u Faculty of Health and Social Studies, University of South Bohemia, Emy Destinove, 37005 Ceske Budejovice, Czech Republic. berger@jcu.cz
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- $a A new screening test of a haemolytic potential is presented on Spodoptera larvae in vivo. The assay was evaluated using phenylhydrazine, the well-known xenobiotic which induces haemolysis in mammals. Haemocyte disintegration causes changes in total haemocyte counts and differentials. It is shown that only qualitative evaluation of panoptically stained haemolymph smears is sufficient, fast and cheap to detect haemolytic potential. The presented assay also prevents unneeded, non-ethical use of vertebrates.
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- $t Basic & Clinical Pharmacology & Toxicology $p Basic Clin Pharmacol Toxicol $g Roč. 105, č. 5 (2009), s. 315-318 $w MED00007578 $x 1742-7835
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