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Protective effect of Clostridium tyrobutyricum in acute dextran sodium sulphate-induced colitis: differential regulation of tumour necrosis factor-α and interleukin-18 in BALB/c and severe combined immunodeficiency mice
T. Hudcovic, J. Kolinska, J. Klepetar, R. Stepankova, T. Rezanka, D. Srutkova, M. Schwarzer, V. Erban, Z. Du, JM. Wells, T. Hrncir, H. Tlaskalova-Hogenova, H. Kozakova
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1966 to 1 year ago
PubMed Central
from 1966 to 1 year ago
Medline Complete (EBSCOhost)
from 1966-01-01 to 1 year ago
Wiley Online Library (archiv)
from 1990-01-01 to 2012-12-31
- MeSH
- Acute Disease MeSH
- CD11b Antigen biosynthesis genetics MeSH
- Administration, Rectal MeSH
- Bacterial Translocation MeSH
- Butyrates metabolism MeSH
- Clostridium tyrobutyricum physiology MeSH
- Phosphoproteins biosynthesis genetics MeSH
- Immunocompetence MeSH
- Interleukin-18 biosynthesis genetics MeSH
- Colon metabolism microbiology pathology MeSH
- Fatty Acids metabolism MeSH
- Membrane Proteins biosynthesis genetics MeSH
- Mucin-2 biosynthesis genetics MeSH
- Mucins biosynthesis MeSH
- Mice, Inbred BALB C MeSH
- Mice, SCID MeSH
- Mice MeSH
- Specific Pathogen-Free Organisms MeSH
- Probiotics therapeutic use MeSH
- Dextran Sulfate toxicity MeSH
- Severe Combined Immunodeficiency genetics immunology MeSH
- Tumor Necrosis Factor-alpha biosynthesis genetics MeSH
- Colitis, Ulcerative chemically induced genetics immunology microbiology pathology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
One of the promising approaches in the therapy of ulcerative colitis is administration of butyrate, an energy source for colonocytes, into the lumen of the colon. This study investigates the effect of butyrate producing bacterium Clostridium tyrobutyricum on dextran sodium sulphate (DSS)-induced colitis in mice. Immunocompetent BALB/c and immunodeficient severe combined immunodeficiency (SCID) mice reared in specific-pathogen-free (SPF) conditions were treated intrarectally with C. tyrobutyricum 1 week prior to the induction of DSS colitis and during oral DSS treatment. Administration of DSS without C. tyrobutyricum treatment led to an appearance of clinical symptoms - bleeding, rectal prolapses and colitis-induced increase in the antigen CD11b, a marker of infiltrating inflammatory cells in the lamina propria. The severity of colitis was similar in BALB/c and SCID mice as judged by the histological damage score and colon shortening after 7 days of DSS treatment. Both strains of mice also showed a similar reduction in tight junction (TJ) protein zonula occludens (ZO)-1 expression and of MUC-2 mucin depression. Highly elevated levels of cytokine tumour necrosis factor (TNF)-α in the colon of SCID mice and of interleukin (IL)-18 in BALB/c mice were observed. Intrarectal administration of C. tyrobutyricum prevented appearance of clinical symptoms of DSS-colitis, restored normal MUC-2 production, unaltered expression of TJ protein ZO-1 and decreased levels of TNF-α and IL-18 in the descending colon of SCID and BALB/c mice, respectively. Some of these features can be ascribed to the increased production of butyrate in the lumen of the colon and its role in protection of barrier functions and regulation of IL-18 expression.
Food Research Institute Prague Czech Republic
Host Microbe Interactomics Animal Sciences Wageningen University Wageningen The Netherlands
Institute of Microbiology of Academy of Sciences of Czech Republic v v i Prague Czech Republic
Institute of Microbiology of the Academy of Sciences of the Czech Republic v v i Prague
Institute of Physiology of the Academy of Sciences of the Czech Republic v v i Prague
References provided by Crossref.org
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