-
Something wrong with this record ?
Primary rat hepatocytes in chemical testing and QSAR predictive applicability
M. Tichý, A. Pokorná, I. Hanzlíková, J. Nerudová, J. Tumová, R. Uzlová
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Coloring Agents MeSH
- Chemistry, Physical MeSH
- Hepatocytes drug effects metabolism MeSH
- Indicators and Reagents MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Quantitative Structure-Activity Relationship MeSH
- Urea metabolism MeSH
- Rats, Wistar MeSH
- Predictive Value of Tests MeSH
- Solutions MeSH
- Cell Separation MeSH
- Toxicology methods MeSH
- Trypan Blue MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Primary rat hepatocytes were used to test acute toxicities of 16 neutral aliphatic alcohols, ketones and esters. Their effects on cell viability and metabolic function (ureogenesis, i.e. biotransformation of ornithine to urea) were measured and expressed as EC50 values. Log EC50 values from both tests correlated with the log partition coefficients for the chemicals between n-octanol and water and log P(ow)-based QSAR models were derived. Log EC50 (viability) tightly correlates with log EC50 (ureogenesis): log EC50 (viability)=0.91 log EC50 (ureogenesis)+0.06. Each of these toxic indices can be substituted by the other one. The toxic indices for both cell viability and metabolic disorder can be estimated using log EC50 for movement inhibition in the oligochaete Tubifex tubifex and the respective QSAR equation. It eliminates a usage of rats. Their correlations were proved and justified.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12025539
- 003
- CZ-PrNML
- 005
- 20130123012621.0
- 007
- ta
- 008
- 120816s2010 enk f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.tiv.2009.08.028 $2 doi
- 035 __
- $a (PubMed)19735719
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Tichý, Miloň, $d 1937- $7 jn20000402359 $u National Institute of Public Health, Praha 2, Czech Republic. mtichy@szu.cz
- 245 10
- $a Primary rat hepatocytes in chemical testing and QSAR predictive applicability / $c M. Tichý, A. Pokorná, I. Hanzlíková, J. Nerudová, J. Tumová, R. Uzlová
- 520 9_
- $a Primary rat hepatocytes were used to test acute toxicities of 16 neutral aliphatic alcohols, ketones and esters. Their effects on cell viability and metabolic function (ureogenesis, i.e. biotransformation of ornithine to urea) were measured and expressed as EC50 values. Log EC50 values from both tests correlated with the log partition coefficients for the chemicals between n-octanol and water and log P(ow)-based QSAR models were derived. Log EC50 (viability) tightly correlates with log EC50 (ureogenesis): log EC50 (viability)=0.91 log EC50 (ureogenesis)+0.06. Each of these toxic indices can be substituted by the other one. The toxic indices for both cell viability and metabolic disorder can be estimated using log EC50 for movement inhibition in the oligochaete Tubifex tubifex and the respective QSAR equation. It eliminates a usage of rats. Their correlations were proved and justified.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a separace buněk $7 D002469
- 650 _2
- $a kultivované buňky $7 D002478
- 650 _2
- $a fyzikální chemie $7 D002627
- 650 _2
- $a barvicí látky $7 D004396
- 650 _2
- $a vztah mezi dávkou a účinkem léčiva $7 D004305
- 650 _2
- $a hepatocyty $x účinky léků $x metabolismus $7 D022781
- 650 _2
- $a indikátory a reagencie $7 D007202
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a prediktivní hodnota testů $7 D011237
- 650 _2
- $a kvantitativní vztahy mezi strukturou a aktivitou $7 D021281
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a potkani Wistar $7 D017208
- 650 _2
- $a roztoky $7 D012996
- 650 _2
- $a toxikologie $x metody $7 D014116
- 650 _2
- $a trypanová modř $7 D014343
- 650 _2
- $a močovina $x metabolismus $7 D014508
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Pokorná, Adéla, $d 1972- $7 xx0101834
- 700 1_
- $a Hanzlíková, Iveta $7 xx0114170
- 700 1_
- $a Nerudová, Jana $7 xx0079542
- 700 1#
- $a Tumová, Jana. $7 _AN041464
- 700 1#
- $a Uzlová, Rút. $7 _AN062062
- 773 0_
- $w MED00004536 $t Toxicology in vitro : an international journal published in association with BIBRA $x 1879-3177 $g Roč. 24, č. 1 (2010), s. 240-244
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/19735719 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120816 $b ABA008
- 991 __
- $a 20130123012805 $b ABA008
- 999 __
- $a ok $b bmc $g 947581 $s 782885
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2010 $b 24 $c 1 $d 240-244 $i 1879-3177 $m Toxicology in vitro $n Toxicol In Vitro $x MED00004536
- LZP __
- $a Pubmed-20120816/10/02
