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Sample preparation methods for MALDI-MS profiling of bacteria
O. Šedo, I. Sedláček, Z. Zdráhal,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
21500244
DOI
10.1002/mas.20287
Knihovny.cz E-resources
- MeSH
- Bacteria chemistry isolation & purification MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Bacterial Typing Techniques methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
Direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed.
References provided by Crossref.org
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