-
Something wrong with this record ?
Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study
M. Masopust, Z. Vykoukalová, A. Knoll, H. Bartenschlager, A. Mileham, N. Deeb, GA. Rohrer, S. Cepica,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2011-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 1 year ago
- MeSH
- Genome-Wide Association Study MeSH
- DNA Primers genetics MeSH
- Phenotype MeSH
- Polymorphism, Single Nucleotide genetics MeSH
- Cloning, Molecular MeSH
- Linear Models MeSH
- Chromosome Mapping MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Swine genetics MeSH
- Insulin Receptor Substrate Proteins genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology MeSH
- Body Weights and Measures MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96C
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12027922
- 003
- CZ-PrNML
- 005
- 20121210094153.0
- 007
- ta
- 008
- 120817s2011 ne f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1007/s11033-010-0402-x $2 doi
- 035 __
- $a (PubMed)21104145
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Masopust, Martin $u Institute of Animal Physiology and Genetics AS CR, v.v.i., Liběchov, Czech Republic.
- 245 10
- $a Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study / $c M. Masopust, Z. Vykoukalová, A. Knoll, H. Bartenschlager, A. Mileham, N. Deeb, GA. Rohrer, S. Cepica,
- 520 9_
- $a Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96C<G in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829T<C in the coding sequence with allele C present only in Meishan. Linkage mapping placed the IRS4 gene at position 82 cM on the current USDA-USMARC linkage map of porcine chromosome X. Association analyses were performed on 555 animals of 12th-15th generation of the Meishan × Large White cross and showed that both SNPs were highly significantly associated with backfat depth (P=0.0005) and that the SNP FN424076:g1829T<C was also associated with loin depth (P=0.017). The Meishan alleles increased back fat depth and decreased loin depth. IRS4 can be considered a positional candidate gene for at least some of the QTL located at the centromeric region of porcine chromosome X.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a sekvence nukleotidů $7 D001483
- 650 _2
- $a tělesné váhy a míry $7 D001837
- 650 _2
- $a mapování chromozomů $7 D002874
- 650 _2
- $a klonování DNA $7 D003001
- 650 _2
- $a DNA primery $x genetika $7 D017931
- 650 _2
- $a celogenomová asociační studie $7 D055106
- 650 _2
- $a proteiny insulinového receptorového substrátu $x genetika $7 D055504
- 650 _2
- $a lineární modely $7 D016014
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a fenotyp $7 D010641
- 650 _2
- $a polymerázová řetězová reakce $7 D016133
- 650 _2
- $a jednonukleotidový polymorfismus $x genetika $7 D020641
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 650 _2
- $a sekvenční homologie $7 D017385
- 650 _2
- $a prasata $x genetika $7 D013552
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Vykoukalová, Zuzana
- 700 1_
- $a Knoll, Aleš
- 700 1_
- $a Bartenschlager, Heinz
- 700 1_
- $a Mileham, Alan
- 700 1_
- $a Deeb, Nader
- 700 1_
- $a Rohrer, Gary A
- 700 1_
- $a Cepica, Stanislav
- 773 0_
- $w MED00003392 $t Molecular biology reports $x 1573-4978 $g Roč. 38, č. 4 (2011), s. 2611-7
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/21104145 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20121210094230 $b ABA008
- 999 __
- $a ok $b bmc $g 949964 $s 785268
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2011 $b 38 $c 4 $d 2611-7 $i 1573-4978 $m Molecular biology reports $n Mol Biol Rep $x MED00003392
- LZP __
- $a Pubmed-20120817/11/03
