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Transactivation by temperature-dependent p53 mutants in yeast and human cells
J. Slovackova, D. Grochova, J. Navratilova, J. Smarda, J. Smardova,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NS10448
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
Free Medical Journals
from 2002 to 1 year ago
PubMed Central
from 2009 to 1 year ago
Europe PubMed Central
from 2009 to 1 year ago
PubMed
20505364
DOI
10.4161/cc.9.11.11808
Knihovny.cz E-resources
- MeSH
- Transcriptional Activation MeSH
- Humans MeSH
- Mutation MeSH
- Cell Line, Tumor MeSH
- Tumor Suppressor Protein p53 genetics metabolism MeSH
- Saccharomyces cerevisiae genetics metabolism MeSH
- Gene Expression Profiling MeSH
- Temperature MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The p53 protein plays an important role in cancer prevention. In response to stress signals, p53 controls essential cell functions by regulating expression of its target genes. Full or partial loss of the p53 function in cancer cells usually results from mutations of the p53 gene. Some of them are temperature-dependent, allowing reactivation of the p53 function in certain temperature. These mutations can alter general transactivation ability of the p53 protein or they modify its transactivation only towards specific genes. We analyzed transactivation of several target genes by 23 temperature-dependent p53 mutants and stratified them into four functional groups. Seventeen p53 mutants exhibited temperature-dependency and discriminative character in human and yeast cells. Despite the differences of yeast and human cells, they allowed similar transactivation rates to the p53 mutants, thus providing evidence that functional analysis of separated alleles in yeast is valuable tool for assessment of the human p53 status.
References provided by Crossref.org
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