-
Je něco špatně v tomto záznamu ?
Regioisomer separation and identification of triacylglycerols containing vaccenic and oleic acids, and α- and γ-linolenic acids, in thermophilic cyanobacteria Mastigocladus laminosus and Tolypothrix sp
T. Řezanka, J. Lukavský, L. Siristova, K. Sigler,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- isomerie MeSH
- kyselina gama-linolenová analýza MeSH
- kyseliny olejové analýza MeSH
- molekulární struktura MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- sinice chemie MeSH
- triglyceridy analýza MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/APCI-MS) was used for direct analysis of triacylglycerols (TAGs) from different strains of the cyanobacteria Mastigocladus laminosus, Tolypothrix cf. tenuis and Tolypothrix distorta. This technique enabled us to identify and quantify the specific molecular species of TAGs directly from lipid extracts of the cyanobacteria. The regioisomeric series of TAGs having α-linolenic and γ-linolenic and also oleic and cis-vaccenic acids were separated by RP-HPLC and identified by APCI-MS. M. laminosus produced only a few molecular species of TAGs, including both isomers of octadecenoic (oleic and vaccenic) acid, while T. distorta contained tens of molecular species of TAGs having FAs with up to four double bonds (stearidonic acid and including also its positional isomer, i.e. 3,6,9,12-octadecatetraenoic acid) and both positional isomers (α and γ) of linolenic acids. Individual strains of both cyanobacteria exhibited different contents of polyunsaturated fatty acids (Tolypothrix sp.) and different distribution of positional isomers of monoenoic fatty acids in TAGs (M. laminosus).
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12034634
- 003
- CZ-PrNML
- 005
- 20121210110629.0
- 007
- ta
- 008
- 121023s2012 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.phytochem.2012.02.028 $2 doi
- 035 __
- $a (PubMed)22445652
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Řezanka, Tomáš $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic. rezanka@biomed.cas.cz
- 245 10
- $a Regioisomer separation and identification of triacylglycerols containing vaccenic and oleic acids, and α- and γ-linolenic acids, in thermophilic cyanobacteria Mastigocladus laminosus and Tolypothrix sp / $c T. Řezanka, J. Lukavský, L. Siristova, K. Sigler,
- 520 9_
- $a Reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/APCI-MS) was used for direct analysis of triacylglycerols (TAGs) from different strains of the cyanobacteria Mastigocladus laminosus, Tolypothrix cf. tenuis and Tolypothrix distorta. This technique enabled us to identify and quantify the specific molecular species of TAGs directly from lipid extracts of the cyanobacteria. The regioisomeric series of TAGs having α-linolenic and γ-linolenic and also oleic and cis-vaccenic acids were separated by RP-HPLC and identified by APCI-MS. M. laminosus produced only a few molecular species of TAGs, including both isomers of octadecenoic (oleic and vaccenic) acid, while T. distorta contained tens of molecular species of TAGs having FAs with up to four double bonds (stearidonic acid and including also its positional isomer, i.e. 3,6,9,12-octadecatetraenoic acid) and both positional isomers (α and γ) of linolenic acids. Individual strains of both cyanobacteria exhibited different contents of polyunsaturated fatty acids (Tolypothrix sp.) and different distribution of positional isomers of monoenoic fatty acids in TAGs (M. laminosus).
- 650 _2
- $a vysokoúčinná kapalinová chromatografie $x metody $7 D002851
- 650 _2
- $a sinice $x chemie $7 D000458
- 650 _2
- $a plynová chromatografie s hmotnostně spektrometrickou detekcí $7 D008401
- 650 _2
- $a isomerie $7 D007536
- 650 _2
- $a molekulární struktura $7 D015394
- 650 _2
- $a kyseliny olejové $x analýza $7 D009829
- 650 _2
- $a triglyceridy $x analýza $7 D014280
- 650 _2
- $a kyselina gama-linolenová $x analýza $7 D017965
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Lukavský, Jaromír
- 700 1_
- $a Siristova, Lucie
- 700 1_
- $a Sigler, Karel
- 773 0_
- $w MED00003829 $t Phytochemistry $x 1873-3700 $g Roč. 78(2012), s. 147-55
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22445652 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a
- 990 __
- $a 20121023 $b ABA008
- 991 __
- $a 20121210110705 $b ABA008
- 999 __
- $a ok $b bmc $g 956644 $s 792131
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2012 $b 78 $d 147-55 $i 1873-3700 $m Phytochemistry $n Phytochemistry $x MED00003829
- LZP __
- $a Pubmed-20121023
