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Thin-layer hydroxyapatite deposition on a nanofiber surface stimulates mesenchymal stem cell proliferation and their differentiation into osteoblasts
E. Prosecká, M. Buzgo, M. Rampichová, T. Kocourek, P. Kochová, L. Vysloužilová, D. Tvrdík, M. Jelínek, D. Lukáš, E. Amler,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT12156
MZ0
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Full text - Article
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ROAD: Directory of Open Access Scholarly Resources
from 2000 to 2012
PubMed
22319242
DOI
10.1155/2012/428503
Knihovny.cz E-resources
- MeSH
- Cell Differentiation drug effects MeSH
- Durapatite chemistry pharmacology MeSH
- Mesenchymal Stem Cells cytology MeSH
- Nanofibers chemistry MeSH
- Osteoblasts cytology MeSH
- Polyesters chemistry MeSH
- Polyvinyl Alcohol chemistry MeSH
- Swine MeSH
- Cell Proliferation drug effects MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ɛ-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
References provided by Crossref.org
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