-
Je něco špatně v tomto záznamu ?
Investigation of sanguinarine and chelerythrine effects on LPS-induced inflammatory gene expression in THP-1 cell line
K. Pěnčíková, P. Kollár, V. Müller Závalová, E. Táborská, J. Urbanová, J. Hošek,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antiflogistika farmakologie terapeutické užití MeSH
- benzofenantridiny farmakologie terapeutické užití MeSH
- buněčné linie MeSH
- chemokin CCL2 genetika metabolismus MeSH
- exprese genu účinky léků MeSH
- fytoterapie MeSH
- genetická transkripce účinky léků MeSH
- interleukin-6 genetika metabolismus MeSH
- isochinoliny farmakologie terapeutické užití MeSH
- lidé MeSH
- lipopolysacharidy MeSH
- makrofágy účinky léků metabolismus MeSH
- mediátory zánětu metabolismus MeSH
- prednison farmakologie terapeutické užití MeSH
- rostlinné extrakty farmakologie terapeutické užití MeSH
- zánět farmakoterapie genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Quaternary benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine have been used in folk medicine for their wide range of useful properties. One of their major effect is also anti-inflammatory activity, that is not clarified in detail. This study focused on the ability of these alkaloids to modulate the gene expression of pro-inflammatory tumour necrosis factor α (TNF-α), monocyte chemoattractant protein 1 (MCP-1, also known as CCL-2), interleukin (IL)-6, IL-1β and anti-inflammatory cytokines IL-1 receptor antagonist (IL-1RA) and IL-10. The effect of these alkaloids was compared with that of conventional drug prednisone. Human monocyte-derived macrophages were pre-treated with alkaloids or prednisone and inflammatory reaction was induced by lipopolysaccharide. Changes of gene expression at the transcriptional level of mentioned cytokines were measured. In our study mainly affected pro-inflammatory cytokines were CCL-2 and IL-6. Two hours after LPS stimulation, cells influenced by sanguinarine and chelerythrine significantly declined the CCL-2 expression by a factors of 3.5 (p<0.001) and 1.9 (p<0.01); for those treated with prednisone the factor was 5.3 (p<0.001). Eight hours after LPS induction, both alkaloids significantly diminished the CCL-2 expression. The lower expression was found for sanguinarine--lower by a factor of 4.3 than for cells treated with the vehicle (p<0.001). Two hours after LPS stimulation, cells treated with sanguinarine decreased the IL-6 mRNA level by a factor of 3.9 (p<0.001) compared with cells treated with the vehicle. Chelerythrine decreased the level of IL-6 mRNA by a factor of 1.6 (p<0.001). Sanguinarine decreased gene expression of CCL-2 and IL-6 more than chelerythrine and its effect was quite similar to prednisone. Four hours after LPS stimulation, cells pre-treated with sanguinarine exhibited significantly higher expression (a factor of 1.7, p<0.001) of IL-1RA than cells without sanguinarine treatment. Our results help to clarify possible mechanisms of action of these alkaloids in the course of inflammation.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13000804
- 003
- CZ-PrNML
- 005
- 20130111093814.0
- 007
- ta
- 008
- 130108s2012 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.phymed.2012.04.001 $2 doi
- 035 __
- $a (PubMed)22592163
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Pěnčíková, K $u Department of Biochemistry, Faculty of Medicine, Masaryk University, Kamenice 5/A16, 62500 Brno, Czech Republic. k.pencikova@mail.muni.cz
- 245 10
- $a Investigation of sanguinarine and chelerythrine effects on LPS-induced inflammatory gene expression in THP-1 cell line / $c K. Pěnčíková, P. Kollár, V. Müller Závalová, E. Táborská, J. Urbanová, J. Hošek,
- 520 9_
- $a Quaternary benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine have been used in folk medicine for their wide range of useful properties. One of their major effect is also anti-inflammatory activity, that is not clarified in detail. This study focused on the ability of these alkaloids to modulate the gene expression of pro-inflammatory tumour necrosis factor α (TNF-α), monocyte chemoattractant protein 1 (MCP-1, also known as CCL-2), interleukin (IL)-6, IL-1β and anti-inflammatory cytokines IL-1 receptor antagonist (IL-1RA) and IL-10. The effect of these alkaloids was compared with that of conventional drug prednisone. Human monocyte-derived macrophages were pre-treated with alkaloids or prednisone and inflammatory reaction was induced by lipopolysaccharide. Changes of gene expression at the transcriptional level of mentioned cytokines were measured. In our study mainly affected pro-inflammatory cytokines were CCL-2 and IL-6. Two hours after LPS stimulation, cells influenced by sanguinarine and chelerythrine significantly declined the CCL-2 expression by a factors of 3.5 (p<0.001) and 1.9 (p<0.01); for those treated with prednisone the factor was 5.3 (p<0.001). Eight hours after LPS induction, both alkaloids significantly diminished the CCL-2 expression. The lower expression was found for sanguinarine--lower by a factor of 4.3 than for cells treated with the vehicle (p<0.001). Two hours after LPS stimulation, cells treated with sanguinarine decreased the IL-6 mRNA level by a factor of 3.9 (p<0.001) compared with cells treated with the vehicle. Chelerythrine decreased the level of IL-6 mRNA by a factor of 1.6 (p<0.001). Sanguinarine decreased gene expression of CCL-2 and IL-6 more than chelerythrine and its effect was quite similar to prednisone. Four hours after LPS stimulation, cells pre-treated with sanguinarine exhibited significantly higher expression (a factor of 1.7, p<0.001) of IL-1RA than cells without sanguinarine treatment. Our results help to clarify possible mechanisms of action of these alkaloids in the course of inflammation.
- 650 _2
- $a antiflogistika $x farmakologie $x terapeutické užití $7 D000893
- 650 _2
- $a benzofenantridiny $x farmakologie $x terapeutické užití $7 D053119
- 650 _2
- $a buněčné linie $7 D002460
- 650 _2
- $a chemokin CCL2 $x genetika $x metabolismus $7 D018932
- 650 _2
- $a exprese genu $x účinky léků $7 D015870
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a zánět $x farmakoterapie $x genetika $x metabolismus $7 D007249
- 650 _2
- $a mediátory zánětu $x metabolismus $7 D018836
- 650 _2
- $a interleukin-6 $x genetika $x metabolismus $7 D015850
- 650 _2
- $a isochinoliny $x farmakologie $x terapeutické užití $7 D007546
- 650 _2
- $a lipopolysacharidy $7 D008070
- 650 _2
- $a makrofágy $x účinky léků $x metabolismus $7 D008264
- 650 _2
- $a fytoterapie $7 D008517
- 650 _2
- $a rostlinné extrakty $x farmakologie $x terapeutické užití $7 D010936
- 650 _2
- $a prednison $x farmakologie $x terapeutické užití $7 D011241
- 650 _2
- $a genetická transkripce $x účinky léků $7 D014158
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kollár, P
- 700 1_
- $a Müller Závalová, V
- 700 1_
- $a Táborská, E
- 700 1_
- $a Urbanová, J
- 700 1_
- $a Hošek, J
- 773 0_
- $w MED00003830 $t Phytomedicine : international journal of phytotherapy and phytopharmacology $x 1618-095X $g Roč. 19, č. 10 (2012), s. 890-5
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22592163 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20130108 $b ABA008
- 991 __
- $a 20130111093921 $b ABA008
- 999 __
- $a ok $b bmc $g 963586 $s 798968
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2012 $b 19 $c 10 $d 890-5 $i 1618-095X $m Phytomedicine $n Phytomedicine $x MED00003830
- LZP __
- $a Pubmed-20130108
