Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Expression of CDK7/CAK in normal and tumor cells of diverse histogenesis, cell-cycle position and differentiation

J Bartkova, M Zemanova, J Bartek

. 1996 ; 66 (6) : 732-737.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu srovnávací studie, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc13017772

Grantová podpora
IZ1919 MZ0 CEP - Centrální evidence projektů

The cyclin-dependent kinase 7 (CDK7) represents the 40-kDa catalytic subunit of the CDK-activating kinase, the enzyme responsible for activatory phosphorylation of multiple CDKs controlling G1, S and G2/M phases of the cell cycle. Here, we surveyed a wide range of normal and tumour cell types, in both cell culture and biopsy specimens, for abundance and subcellular localisation of the CDK7 protein. Immunoblotting and immunocytochemical analyses showed that CDK7 was (i) ubiquitously expressed in all cell types examined; (ii) exclusively nuclear; (iii) moderately elevated in tumour cells when compared with their normal cell counterparts; (iv) invariant throughout the cell cycle of normal lymphocytes, fibroblasts, breast epithelium and several cancer cell lines; and (v) clearly detectable even in quiescent cells, including highly differentiated cell types in situ. Our data are consistent with the emerging role for CDK7/CAK in multiple biological processes, possibly providing a link between cell-cycle control, transcriptional regulation and genomic integrity control.

000      
00000naa a2200000 a 4500
001      
bmc13017772
003      
CZ-PrNML
005      
20130514090351.0
007      
ta
008      
130509s1996 xxuad f 000 0|eng||
009      
AR
035    __
$a (PubMed)8647641
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Bártková, Jiřina $7 xx0094304 $u Division for Cancer Biology, Danish Cancer Society, Copenhagen, Denmark.
245    10
$a Expression of CDK7/CAK in normal and tumor cells of diverse histogenesis, cell-cycle position and differentiation / $c J Bartkova, M Zemanova, J Bartek
520    9_
$a The cyclin-dependent kinase 7 (CDK7) represents the 40-kDa catalytic subunit of the CDK-activating kinase, the enzyme responsible for activatory phosphorylation of multiple CDKs controlling G1, S and G2/M phases of the cell cycle. Here, we surveyed a wide range of normal and tumour cell types, in both cell culture and biopsy specimens, for abundance and subcellular localisation of the CDK7 protein. Immunoblotting and immunocytochemical analyses showed that CDK7 was (i) ubiquitously expressed in all cell types examined; (ii) exclusively nuclear; (iii) moderately elevated in tumour cells when compared with their normal cell counterparts; (iv) invariant throughout the cell cycle of normal lymphocytes, fibroblasts, breast epithelium and several cancer cell lines; and (v) clearly detectable even in quiescent cells, including highly differentiated cell types in situ. Our data are consistent with the emerging role for CDK7/CAK in multiple biological processes, possibly providing a link between cell-cycle control, transcriptional regulation and genomic integrity control.
590    __
$a bohemika - dle Pubmed
650    02
$a prsy $x cytologie $x metabolismus $7 D001940
650    12
$a buněčný cyklus $x genetika $7 D002453
650    02
$a buněčná diferenciace $x genetika $x účinky léků $7 D002454
650    02
$a buněčné jádro $x metabolismus $7 D002467
650    12
$a cyklin-dependentní kinasy $7 D018844
650    02
$a ženské pohlaví $7 D005260
650    02
$a fibroblasty $x metabolismus $7 D005347
650    12
$a regulace genové exprese $7 D005786
650    02
$a regulace genové exprese u nádorů $7 D015972
650    02
$a lidé $7 D006801
650    02
$a lymfocyty $x metabolismus $7 D008214
650    12
$a nádorové proteiny $x biosyntéza $x genetika $7 D009363
650    02
$a nádory $x genetika $x metabolismus $x patologie $7 D009369
650    12
$a nádorové kmenové buňky $x metabolismus $7 D014411
650    12
$a protein-serin-threoninkinasy $x biosyntéza $x genetika $7 D017346
650    02
$a retina $x cytologie $x embryologie $x metabolismus $7 D012160
650    02
$a genetická transkripce $7 D014158
655    _2
$a srovnávací studie $7 D003160
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Zemanová, Marie $u Institute of hematology and Blood Transfusion, Prague, Czech Republic
700    1_
$a Bártek, Jiří, $d 1953- $7 xx0046271 $u Division for Cancer Biology, Danish Cancer Society, Copenhagen, Denmark.
773    0_
$t International Journal of Cancer $x 0020-7136 $g Roč. 66, č. 6 (1996), s. 732-737 $p Int J Cancer $w MED00002298
910    __
$a ABA008 $b A 3679 $y 3 $z 0
990    __
$a 20130509164730 $b ABA008
991    __
$a 20130514090702 $b ABA008
999    __
$a ok $b bmc $g 981120 $s 816105
BAS    __
$a 3
BMC    __
$x MED00002298 $i 0020-7136 $a 1996 $b 66 $c 6 $d 732-737 $m International journal of cancer $n Int J Cancer
GRA    __
$a IZ1919 $p MZ0
LZP    __
$a NLK 2013-05/lpbo

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace