Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Isolation and characterization of human CXCR4-positive pancreatic cells

T Koblas, K Zacharovova, Z Berkova, M Mindlova, P Girman, E Dovolilova, L Karasova, F Saudek

. 2007 ; 53 (1) : 13-22.

Language English Country Czech Republic

Document type Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc13027017

Grant support
NR8031 MZ0 CEP Register

The existence of an adult PSC that may be used in the treatment of diabetes is still a matter of scientific debate as conclusive evidence of such a stem cell in the adult pancreas has not yet been presented. The main reason why putative PSC has not yet been identified is the lack of specific markers that may be used to isolate and purify them. In order to increase the list of potential PSC markers we have focused on the human pancreatic cells that express cell surface receptor CXCR4, a marker of stem cells derived from different adult tissues. Here we report that CXCR4-positive pancreatic cells express markers of pancreatic endocrine progenitors (neurogenin-3, nestin) and markers of pluripotent stem cells (Oct-4, Nanog, ABCG2, CD133, CD117). Upon in vitro differentiation, these cells form ILCC and produce key islet hormones including insulin. Based on our results, we assume that CXCR4 marks pancreatic endocrine progenitors and in combination with other cell surface markers may be used in the attempt to identify and isolate PSC.

000      
00000naa a2200000 a 4500
001      
bmc13027017
003      
CZ-PrNML
005      
20130819141411.0
007      
ta
008      
130819s2007 xr f 000 0|eng||
009      
AR
035    __
$a (PubMed)17328838
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Koblas, Tomáš $7 xx0070806 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
245    10
$a Isolation and characterization of human CXCR4-positive pancreatic cells / $c T Koblas, K Zacharovova, Z Berkova, M Mindlova, P Girman, E Dovolilova, L Karasova, F Saudek
520    9_
$a The existence of an adult PSC that may be used in the treatment of diabetes is still a matter of scientific debate as conclusive evidence of such a stem cell in the adult pancreas has not yet been presented. The main reason why putative PSC has not yet been identified is the lack of specific markers that may be used to isolate and purify them. In order to increase the list of potential PSC markers we have focused on the human pancreatic cells that express cell surface receptor CXCR4, a marker of stem cells derived from different adult tissues. Here we report that CXCR4-positive pancreatic cells express markers of pancreatic endocrine progenitors (neurogenin-3, nestin) and markers of pluripotent stem cells (Oct-4, Nanog, ABCG2, CD133, CD117). Upon in vitro differentiation, these cells form ILCC and produce key islet hormones including insulin. Based on our results, we assume that CXCR4 marks pancreatic endocrine progenitors and in combination with other cell surface markers may be used in the attempt to identify and isolate PSC.
590    __
$a bohemika - dle Pubmed
650    02
$a dospělí $7 D000328
650    02
$a senioři $7 D000368
650    02
$a C-peptid $x metabolismus $7 D002096
650    02
$a buněčná diferenciace $7 D002454
650    12
$a separace buněk $7 D002469
650    02
$a kultivované buňky $7 D002478
650    02
$a fluorescenční protilátková technika $7 D005455
650    02
$a regulace genové exprese $7 D005786
650    02
$a glukagon $x metabolismus $7 D005934
650    02
$a lidé $7 D006801
650    02
$a inzulin $x metabolismus $7 D007328
650    02
$a proteiny intermediálních filament $x metabolismus $7 D007381
650    02
$a receptor leukemického inhibičního faktoru - alfa-podjednotka $x metabolismus $7 D053680
650    02
$a lidé středního věku $7 D008875
650    02
$a proteiny nervové tkáně $x metabolismus $7 D009419
650    12
$a pankreas $x cytologie $7 D010179
650    02
$a messenger RNA $x genetika $x metabolismus $7 D012333
650    02
$a receptory CXCR4 $x genetika $x metabolismus $7 D019718
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Zacharovová, Klára $7 xx0074471 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Berková, Zuzana $7 xx0081890 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Mindlová, Martina $7 xx0092588 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Girman, Peter $7 xx0081892 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Dovolilová, Eva $7 xx0077055 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Karasová, Ludmila $7 xx0061180 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Saudek, František, $d 1955- $7 nlk20000083698 $u Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
773    0_
$t Folia Biologica $x 0015-5500 $g Roč. 53, č. 1 (2007), s. 13-22 $p Folia Biol (Praha) $w MED00011004
856    41
$u https://fb.cuni.cz/Data/files/folia_biologica/volume_53_2007_1/FB2007A0003.pdf $y plný text volně přístupný
910    __
$a ABA008 $b A 970 $y 3 $z 0
990    __
$a 20130819083454 $b ABA008
991    __
$a 20130819141925 $b ABA008
999    __
$a ok $b bmc $g 990934 $s 825441
BAS    __
$a 3
BMC    __
$x MED00011004 $i 0015-5500 $a 2007 $b 53 $c 1 $d 13-22 $m Folia biologica (Praha) $n Folia biol. (Praha)
GRA    __
$a NR8031 $p MZ0
LZP    __
$a NLK 201308/lpbo

Find record

Citation metrics

Archiving options