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Meloxicam synergistically enhances the in vitro effects of sunitinib malate on bladder-cancer cells
Regina Arantes-Rodrigues, Rosario Pinto-Leite, Lio Fidalgo-Goncalves, Isabel Gaivao, Aura Colaco, Paula Oliveira, Lucio Santos
Jazyk angličtina Země Česko
Typ dokumentu srovnávací studie, práce podpořená grantem
NLK
Free Medical Journals
od 2003 do 2013
Freely Accessible Science Journals
od 2003 do 2013
ROAD: Directory of Open Access Scholarly Resources
od 2002
- MeSH
- antiflogistika nesteroidní terapeutické užití MeSH
- apoptóza MeSH
- autofagie MeSH
- buněčné linie cytologie účinky léků MeSH
- fluorescenční mikroskopie MeSH
- indoly terapeutické užití MeSH
- inhibitory angiogeneze * terapeutické užití MeSH
- inhibitory cyklooxygenasy * terapeutické užití MeSH
- kombinovaná farmakoterapie MeSH
- kometový test statistika a číselné údaje MeSH
- nádorové buněčné linie cytologie účinky léků MeSH
- nádory močového měchýře * farmakoterapie patologie MeSH
- poškození DNA účinky léků MeSH
- proliferace buněk účinky léků MeSH
- protinádorové látky terapeutické užití MeSH
- pyrroly terapeutické užití MeSH
- statistika jako téma MeSH
- synergismus léků MeSH
- techniky in vitro MeSH
- thiaziny terapeutické užití MeSH
- thiazoly terapeutické užití MeSH
- Publikační typ
- práce podpořená grantem MeSH
- srovnávací studie MeSH
To evaluate the in vitro effects of sunitinib malate and meloxicam in isolation, and to analyse the ability of meloxicam to enhance the cytotoxicity of sunitinib malate in three human bladder-cancer cell lines. Cell lines were treated with sunitinib malate and meloxicam, either in isolation or combined. Leishman staining, MTT method, comet assay, MDC staining and M30 CytoDEATH antibody were performed. The Chou and Talalay method was applied. Sunitinib malate and meloxicam supressed cell proliferation in bladder-cancer cells in isolation, in a concentration-dependent manner. Treatment of bladder-cancer cells with a combination of sunitinib malate and meloxicam showed a synergistic effect. When exploring the mechanism of this combination by means of comet assay, there is the suggestion that meloxicam increases sunitinib malate cytotoxicity through DNA damage. Autophagic and apoptotic studies show a greater incidence of autophagic vacuoles and early apoptotic cells when the combined treatment was put into use. In isolation, sunitinib malate and meloxicam demonstrated anti-tumour effects in our study. Furthermore, simultaneous exposure of cells to sunitinib malate and meloxicam provided a combinatorial beneficial effect. This hints at the possibility of a new combined therapeutic regimen, which could lead to improvements in the treatment of patients with bladder cancer.
Department of Engineering CMUTAD University of Trás os Montes and Alto Douro Vila Real Portugal
Experimental Pathology and Therapeutics Group Portuguese Institute of Oncology Porto Portugal
Citace poskytuje Crossref.org
Literatura
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