-
Something wrong with this record ?
An influence of ethanol and temperature on products formation by different preparations of Zymomonas mobilis extracellular levansucrase
A. Vigants, D. Upite, R. Scherbaka, J. Lukjanenko, R. Ionina
Language English Country Czech Republic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Ethanol pharmacology MeSH
- Hexosyltransferases metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Oligosaccharides analysis biosynthesis MeSH
- Temperature MeSH
- Zymomonas enzymology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The ethanol and temperature effects on the ratio between Zymomonas mobilis 113S extracellular levansucrase activities were studied using fermentation broth supernatant, "levan-levansucrase" sediment precipitated by ethanol and highly purified enzyme. The fructooligosaccharide (FOS) production at different temperatures in the presence of ethanol was investigated. An ethanol increases FOS biosynthesis activity part of levansucrase. Especially, this effect was pronounced at lower temperatures (35-40 °C) and using purified levansucrase. The inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase was found. The FOS composition containing mostly 1-kestose, 6-kestose, and neokestose obtained in the presence of different ethanol concentrations was found relative constant, while the changes in the sucrose concentration and temperature gave slight changes in the ratio between 1-kestose and 6-kestose.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13033231
- 003
- CZ-PrNML
- 005
- 20131112094406.0
- 007
- ta
- 008
- 131014s2013 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-012-0185-7 $2 doi
- 035 __
- $a (PubMed)22826021
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Vigants, A $u Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda Boulv. 4, 1586, Riga, Latvia. avigants@lanet.lv
- 245 13
- $a An influence of ethanol and temperature on products formation by different preparations of Zymomonas mobilis extracellular levansucrase / $c A. Vigants, D. Upite, R. Scherbaka, J. Lukjanenko, R. Ionina
- 520 9_
- $a The ethanol and temperature effects on the ratio between Zymomonas mobilis 113S extracellular levansucrase activities were studied using fermentation broth supernatant, "levan-levansucrase" sediment precipitated by ethanol and highly purified enzyme. The fructooligosaccharide (FOS) production at different temperatures in the presence of ethanol was investigated. An ethanol increases FOS biosynthesis activity part of levansucrase. Especially, this effect was pronounced at lower temperatures (35-40 °C) and using purified levansucrase. The inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase was found. The FOS composition containing mostly 1-kestose, 6-kestose, and neokestose obtained in the presence of different ethanol concentrations was found relative constant, while the changes in the sucrose concentration and temperature gave slight changes in the ratio between 1-kestose and 6-kestose.
- 650 _2
- $a ethanol $x farmakologie $7 D000431
- 650 _2
- $a hexosyltransferasy $x metabolismus $7 D006602
- 650 _2
- $a koncentrace vodíkových iontů $7 D006863
- 650 _2
- $a oligosacharidy $x analýza $x biosyntéza $7 D009844
- 650 _2
- $a teplota $7 D013696
- 650 _2
- $a Zymomonas $x enzymologie $7 D017355
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Upite, D $u -
- 700 1_
- $a Scherbaka, R $u -
- 700 1_
- $a Lukjanenko, J $u -
- 700 1_
- $a Ionina, R $u -
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 58, č. 1 (2013), s. 75-80
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22826021 $y Pubmed
- 910 __
- $a ABA008 $b online $c sign $y 4 $z 0
- 990 __
- $a 20131014 $b ABA008
- 991 __
- $a 20131112093843 $b ABA008
- 999 __
- $a ok $b bmc $g 1000235 $s 831684
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2013 $b 58 $c 1 $d 75-80 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $b NLK138 $a Pubmed-20131014
