Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast

J. Vecer, P. Vesela, J. Malinsky, P. Herman,

. 2014 ; 588 (3) : 443-9.

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc14050722

We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14050722
003      
CZ-PrNML
005      
20140409124042.0
007      
ta
008      
140401s2014 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.febslet.2013.11.038 $2 doi
035    __
$a (PubMed)24333335
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Vecer, Jaroslav
245    10
$a Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast / $c J. Vecer, P. Vesela, J. Malinsky, P. Herman,
520    9_
$a We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids.
650    _2
$a buněčná membrána $x chemie $x metabolismus $7 D002462
650    _2
$a difenylhexatrien $x chemie $7 D004161
650    _2
$a nenasycené mastné kyseliny $x chemie $7 D005231
650    _2
$a fluorescenční polarizace $7 D005454
650    _2
$a membránové mikrodomény $x chemie $x metabolismus $7 D021962
650    _2
$a mutace $7 D009154
650    _2
$a Saccharomyces cerevisiae $7 D012441
650    _2
$a fluorescenční spektrometrie $7 D013050
650    _2
$a sfingolipidy $x biosyntéza $x chemie $7 D013107
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Vesela, Petra $u -
700    1_
$a Malinsky, Jan $u -
700    1_
$a Herman, Petr $u -
773    0_
$w MED00001785 $t FEBS letters $x 1873-3468 $g Roč. 588, č. 3 (2014), s. 443-9
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24333335 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20140401 $b ABA008
991    __
$a 20140409124130 $b ABA008
999    __
$a ok $b bmc $g 1017858 $s 849302
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 588 $c 3 $d 443-9 $i 1873-3468 $m FEBS letters $n FEBS Lett $x MED00001785
LZP    __
$a Pubmed-20140401

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace