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Polysome profile analysis--yeast
M. Pospísek, L. Valásek,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- centrifugace - gradient hustoty metody MeSH
- northern blotting metody MeSH
- polyribozomy chemie genetika MeSH
- Saccharomyces cerevisiae chemie cytologie genetika MeSH
- sacharosa chemie MeSH
- western blotting metody MeSH
- Publikační typ
- časopisecké články MeSH
More than one 80S monosome can translate an mRNA molecule at a time producing polysomes. The most widely used method to separate 40S and 60S ribosomal subunits from 80S monosomes and polysomes is a high-velocity centrifugation of whole cell extracts in linear sucrose gradients. This polysome profile analysis technique has been routinely used to monitor translational fitness of cells under a variety of physiological conditions, to investigate functions of initiation factors involved in translation, to reveal defects in ribosome biogenesis, to determine roles of 5' UTR structures on mRNA translatability, and more recently for examination of miRNA-mediated translational repression (see an application of this protocol on Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).
Citace poskytuje Crossref.org
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