-
Something wrong with this record ?
Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC-MS
T. Rezanka, I. Kolouchová, A. Cejková, T. Cajthaml, K. Sigler,
Language English Country Germany
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromatography, Reverse-Phase methods MeSH
- Yeasts chemistry classification metabolism MeSH
- Stereoisomerism MeSH
- Triglycerides chemistry metabolism MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia. Chiral LC-APCI-MS is based on using two columns in series packed with a 3,5-dimethylphenyl carbamate modified β-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated. Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fatty acids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10-25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strains containing <10% palmitoleic acid form preferentially symmetrical TAGs.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc14064021
- 003
- CZ-PrNML
- 005
- 20140707113132.0
- 007
- ta
- 008
- 140704s2013 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/jssc.201300657 $2 doi
- 035 __
- $a (PubMed)23963893
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Rezanka, Tomáš $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
- 245 10
- $a Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC-MS / $c T. Rezanka, I. Kolouchová, A. Cejková, T. Cajthaml, K. Sigler,
- 520 9_
- $a LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia. Chiral LC-APCI-MS is based on using two columns in series packed with a 3,5-dimethylphenyl carbamate modified β-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated. Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fatty acids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10-25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strains containing <10% palmitoleic acid form preferentially symmetrical TAGs.
- 650 _2
- $a vysokoúčinná kapalinová chromatografie $x metody $7 D002851
- 650 _2
- $a chromatografie s reverzní fází $x metody $7 D056148
- 650 _2
- $a stereoizomerie $7 D013237
- 650 _2
- $a triglyceridy $x chemie $x metabolismus $7 D014280
- 650 _2
- $a kvasinky $x chemie $x klasifikace $x metabolismus $7 D015003
- 655 _2
- $a hodnotící studie $7 D023362
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kolouchová, Irena
- 700 1_
- $a Cejková, Alena
- 700 1_
- $a Cajthaml, Tomáš
- 700 1_
- $a Sigler, Karel
- 773 0_
- $w MED00006463 $t Journal of separation science $x 1615-9314 $g Roč. 36, č. 20 (2013), s. 3310-20
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/23963893 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20140704 $b ABA008
- 991 __
- $a 20140707113420 $b ABA008
- 999 __
- $a ok $b bmc $g 1031505 $s 862753
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2013 $b 36 $c 20 $d 3310-20 $i 1615-9314 $m Journal of separation science $n J Sep Sci $x MED00006463
- LZP __
- $a Pubmed-20140704
