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High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study
Joana Rolo, Maria Miragaia, Agata Turlej-Rogacka, Joanna Empel, Ons Bouchami, Nuno A. Faria, Ana Tavares, Waleria Hryniewicz, Ad C. Fluit, Hermínia de Lencastre and the CONCORD Working Group, Oto Melter, Helena Žemličková, Marta Fridrichová
Jazyk angličtina Země Spojené státy americké
Typ dokumentu multicentrická studie, práce podpořená grantem
Grantová podpora
NS9642
MZ0
CEP - Centrální evidence projektů
NT12395
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Plný text - Článek
Zdroj
Zdroj
Free Medical Journals od 2006
Public Library of Science (PLoS) od 2006
PubMed Central od 2006
Europe PubMed Central od 2006
ProQuest Central od 2006-12-01
Open Access Digital Library od 2006-01-01
Open Access Digital Library od 2006-10-01
Open Access Digital Library od 2006-01-01
Medline Complete (EBSCOhost) od 2008-01-01
Nursing & Allied Health Database (ProQuest) od 2006-12-01
Health & Medicine (ProQuest) od 2006-12-01
Public Health Database (ProQuest) od 2006-12-01
ROAD: Directory of Open Access Scholarly Resources od 2006
Odkazy
PubMed
22558099
DOI
10.1371/journal.pone.0034768
Knihovny.cz E-zdroje
- MeSH
- genetická variace * MeSH
- infekce získané v komunitě * epidemiologie mikrobiologie MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus * genetika MeSH
- mikrobiální testy citlivosti MeSH
- polymerázová řetězová reakce MeSH
- populační genetika MeSH
- pulzní gelová elektroforéza MeSH
- stafylokokové infekce * epidemiologie mikrobiologie MeSH
- Staphylococcus aureus * genetika MeSH
- techniky typizace bakterií metody MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- multicentrická studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa MeSH
BACKGROUND: Several studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the origin of methicillin-resistant S. aureus (MRSA) in this continent. METHODS AND FINDINGS: A total of 568 colonization and infection isolates, comprising both MRSA and methicillin-susceptible S. aureus (MSSA), were recovered in 16 European countries, from community and community-onset infections. The genetic background of isolates was characterized by molecular typing techniques (spa typing, pulsed-field gel electrophoresis and multilocus sequence typing) and the presence of PVL and ACME was tested by PCR. MRSA were further characterized by SCCmec typing. We found that 59% of all isolates were associated with community-associated clones. Most MRSA were related with USA300 (ST8-IVa and variants) (40%), followed by the European clone (ST80-IVc and derivatives) (28%) and the Taiwan clone (ST59-IVa and related clonal types) (15%). A total of 83% of MRSA carried Panton-Valentine leukocidin (PVL) and 14% carried the arginine catabolic mobile element (ACME). Surprisingly, we found a high genetic diversity among MRSA clonal types (ST-SCCmec), Simpson's index of diversity = 0.852 (0.788-0.916). Specifically, about half of the isolates carried novel associations between genetic background and SCCmec. Analysis by BURP showed that some CA-MSSA and CA-MRSA isolates were highly related, suggesting a probable local acquisition/loss of SCCmec. CONCLUSIONS: Our results imply that CA-MRSA origin, epidemiology and population structure in Europe is very dissimilar from that of USA.
Department of Medical Microbiology 2nd Faculty of Medicine Charles University Prague Czech Republic
Department of Medical Microbiology University Medical Center Utrecht Utrecht The Netherlands
Laboratory of Microbiology The Rockefeller University New York New York United States of America
Laboratory of the National Bone Marrow Transplantation Centre Bab Saadoun Tunis Tunisia
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