A diagnostics of infectious diseases can be done by the immunologic methods or by the amplification of nucleic acid specific to contagious agent using polymerase chain reaction. However, in transmissible spongiform encephalopathies, the infectious agent, prion protein (PrP(Sc)), has the same sequence of nucleic acids as a naturally occurring protein. The other issue with the diagnosing based on the PrP(Sc) detection is that the pathological form of prion protein is abundant only at late stages of the disease in a brain. Therefore, the diagnostics of prion protein caused diseases represent a sort of challenges as that hosts can incubate infectious prion proteins for many months or even years. Therefore, new in vivo assays for detection of prion proteins and for diagnosis of their relation to neurodegenerative diseases are summarized. Their applicability and future prospects in this field are discussed with particular aim at using quantum dots as fluorescent labels.

Brno Czech Republic EU

Brno University of Technology

Central European Institute of Technology

Department of Chemistry and Biochemistry

Faculty of Agronomy

Mendel University in Brno

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$a Sobrova, Pavlina $u Department of Chemistry and Biochemistry; Faculty of Agronomy; Mendel University in Brno; Brno, Czech Republic EU; Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU.

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$a Quantum dots and prion proteins: is this a new challenge for neurodegenerative diseases imaging / $c P. Sobrova, I. Blazkova, J. Chomoucka, J. Drbohlavova, M. Vaculovicova, P. Kopel, J. Hubalek, R. Kizek, V. Adam,

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$a A diagnostics of infectious diseases can be done by the immunologic methods or by the amplification of nucleic acid specific to contagious agent using polymerase chain reaction. However, in transmissible spongiform encephalopathies, the infectious agent, prion protein (PrP(Sc)), has the same sequence of nucleic acids as a naturally occurring protein. The other issue with the diagnosing based on the PrP(Sc) detection is that the pathological form of prion protein is abundant only at late stages of the disease in a brain. Therefore, the diagnostics of prion protein caused diseases represent a sort of challenges as that hosts can incubate infectious prion proteins for many months or even years. Therefore, new in vivo assays for detection of prion proteins and for diagnosis of their relation to neurodegenerative diseases are summarized. Their applicability and future prospects in this field are discussed with particular aim at using quantum dots as fluorescent labels.

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$a Blazkova, Iva $u Department of Chemistry and Biochemistry; Faculty of Agronomy; Mendel University in Brno; Brno, Czech Republic EU.

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$a Chomoucka, Jana $u Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU.

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$a Drbohlavova, Jana $u Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU.

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$a Vaculovicova, Marketa $u Department of Chemistry and Biochemistry; Faculty of Agronomy; Mendel University in Brno; Brno, Czech Republic EU; Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU.

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$a Kopel, Pavel $u Department of Chemistry and Biochemistry; Faculty of Agronomy; Mendel University in Brno; Brno, Czech Republic EU; Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU.

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$a Hubalek, Jaromir $u Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU.

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$a Kizek, Rene $u Department of Chemistry and Biochemistry; Faculty of Agronomy; Mendel University in Brno; Brno, Czech Republic EU; Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU.

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$a Adam, Vojtěch $u Department of Chemistry and Biochemistry; Faculty of Agronomy; Mendel University in Brno; Brno, Czech Republic EU; Central European Institute of Technology; Brno University of Technology; Brno, Czech Republic EU. $7 xx0064599

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