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Cryogenic grinding of electrospun poly-ε-caprolactone mesh submerged in liquid media

P. Knotek, M. Pouzar, M. Buzgo, B. Krizkova, M. Vlcek, A. Mickova, M. Plencner, J. Navesnik, E. Amler, P. Belina,

. 2012 ; 32 (6) : 1366-74.

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc14074792

In this paper, the treatment of poly-ε-caprolactone (PCL) nano/micro-mesh system by cryogenic grinding and subsequent characterization of obtained product is described. The PCL nano/micro-mesh layer submerged in appropriate liquid was cryogenically ground and obtained particles were characterized employing mainly laser diffraction and scanning electron microscopy (SEM). In the ground sample, different types of particles (fibrous particles, fibrous fragments, agglomerates with and without an internal fibrous structure, lamellae and nanoparticles) were identified, described and quantified. Parameters of cryogenic grinding (weight of sample, type of liquid medium, and influence of sample storage) were optimized to maximize the yield of particles with desired features. The potential of the system for cell scaffolding was demonstrated by cultivation of 3T3 fibroblasts on the produced microparticles.

Citace poskytuje Crossref.org

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$a In this paper, the treatment of poly-ε-caprolactone (PCL) nano/micro-mesh system by cryogenic grinding and subsequent characterization of obtained product is described. The PCL nano/micro-mesh layer submerged in appropriate liquid was cryogenically ground and obtained particles were characterized employing mainly laser diffraction and scanning electron microscopy (SEM). In the ground sample, different types of particles (fibrous particles, fibrous fragments, agglomerates with and without an internal fibrous structure, lamellae and nanoparticles) were identified, described and quantified. Parameters of cryogenic grinding (weight of sample, type of liquid medium, and influence of sample storage) were optimized to maximize the yield of particles with desired features. The potential of the system for cell scaffolding was demonstrated by cultivation of 3T3 fibroblasts on the produced microparticles.
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$a Pouzar, Miloslav $u University of Pardubice, Institute of Environmental and Chemical Engineering, Studentská 573, 530 12 Pardubice, Czech Republic.
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$a Buzgo, Matej $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083,142 20, Prague 4, Czech Republic.
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$a Krizkova, Barbora $u University of Pardubice, Department of Biological and Biochemical Sciences, Studentská 573, 530 12 Pardubice, Czech Republic.
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$a Vlcek, Milan $u Institute of Macromolecular Chemistry, Academy of Sciences of Czech Republic, v.v.i., Heyrovskeho sq. 2, 162 06 Prague, Czech Republic.
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$a Mickova, Andrea $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083,142 20, Prague 4, Czech Republic.
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$a Plencner, Martin $u Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083,142 20, Prague 4, Czech Republic.
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