Here, we describe a method for the combined metabolomic, proteomic, transcriptomic and genomic analysis from one single sample as a major step for multilevel data integration strategies in systems biology. While extracting proteins and DNA, this protocol also allows the separation of metabolites into polar and lipid fractions, as well as RNA fractionation into long and small RNAs, thus allowing a broad range of transcriptional studies. The isolated biomolecules are suitable for analysis with different methods that range from electrophoresis and blotting to state-of-the-art procedures based on mass spectrometry (accurate metabolite profiling, shot-gun proteomics) or massive sequencing technologies (transcript analysis). The low amount of starting tissue, its cost-efficiency compared with the utilization of commercial kits, and its performance over a wide range of plant, microbial, and algal species such as Chlamydomonas, Arabidopsis, Populus, or Pinus, makes this method a universal alternative for multiple molecular isolation from plant tissues.

Department of Biology and CESAM University of Aveiro Aveiro Portugal

Global Change Research Centre Czechglobe Academy of Sciences of the Czech Republic Brno Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc15014326

003      

CZ-PrNML

005      

20150429113901.0

007      

ta

008      

150420s2014 enk f 000 0|eng||

009      

AR

024    7_

$a 10.1111/tpj.12546 $2 doi

035    __

$a (PubMed)24804825

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a enk

100    1_

$a Valledor, Luis $u Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal; Global Change Research Centre-Czechglobe, Academy of Sciences of the Czech Republic, Brno, Czech Republic.

245    12

$a A universal protocol for the combined isolation of metabolites, DNA, long RNAs, small RNAs, and proteins from plants and microorganisms / $c L. Valledor, M. Escandón, M. Meijón, E. Nukarinen, MJ. Cañal, W. Weckwerth,

520    9_

$a Here, we describe a method for the combined metabolomic, proteomic, transcriptomic and genomic analysis from one single sample as a major step for multilevel data integration strategies in systems biology. While extracting proteins and DNA, this protocol also allows the separation of metabolites into polar and lipid fractions, as well as RNA fractionation into long and small RNAs, thus allowing a broad range of transcriptional studies. The isolated biomolecules are suitable for analysis with different methods that range from electrophoresis and blotting to state-of-the-art procedures based on mass spectrometry (accurate metabolite profiling, shot-gun proteomics) or massive sequencing technologies (transcript analysis). The low amount of starting tissue, its cost-efficiency compared with the utilization of commercial kits, and its performance over a wide range of plant, microbial, and algal species such as Chlamydomonas, Arabidopsis, Populus, or Pinus, makes this method a universal alternative for multiple molecular isolation from plant tissues.

650    _2

$a Arabidopsis $x genetika $x metabolismus $7 D017360

650    _2

$a Chlamydomonas reinhardtii $x genetika $x metabolismus $7 D016825

650    _2

$a DNA rostlinná $x izolace a purifikace $7 D018744

650    _2

$a genomika $x metody $7 D023281

650    _2

$a metabolomika $x metody $7 D055432

650    _2

$a borovice $x genetika $x metabolismus $7 D028223

650    _2

$a rostlinné proteiny $x izolace a purifikace $7 D010940

650    12

$a rostliny $x genetika $x metabolismus $7 D010944

650    _2

$a Populus $x genetika $x metabolismus $7 D032107

650    _2

$a proteomika $x metody $7 D040901

650    _2

$a RNA rostlin $x izolace a purifikace $7 D018749

650    _2

$a reprodukovatelnost výsledků $7 D015203

650    _2

$a systémová biologie $x metody $7 D049490

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Escandón, Mónica

700    1_

$a Meijón, Mónica

700    1_

$a Nukarinen, Ella

700    1_

$a Cañal, María Jesús

700    1_

$a Weckwerth, Wolfram

773    0_

$w MED00003838 $t The Plant journal for cell and molecular biology $x 1365-313X $g Roč. 79, č. 1 (2014), s. 173-80

856    41

$u https://pubmed.ncbi.nlm.nih.gov/24804825 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20150420 $b ABA008

991    __

$a 20150429114206 $b ABA008

999    __

$a ok $b bmc $g 1071907 $s 897204

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2014 $b 79 $c 1 $d 173-80 $i 1365-313X $m Plant journal $n Plant J $x MED00003838

LZP    __

$a Pubmed-20150420