-
Something wrong with this record ?
Use of fluorescent staining and flow cytometry for monitoring physiological changes in solventogenic clostridia
P. Patakova, M. Linhova, P. Vykydalova, B. Branska, M. Rychtera, K. Melzoch,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Barbiturates MeSH
- Staining and Labeling methods MeSH
- Bioreactors MeSH
- Clostridium physiology ultrastructure MeSH
- Fermentation * MeSH
- Fluoresceins MeSH
- Fluorescent Dyes MeSH
- Isoxazoles MeSH
- Propidium MeSH
- Flow Cytometry MeSH
- Batch Cell Culture Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Physiological changes in populations of Clostridium beijerinckii and Clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. To estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to be a good choice for both species. During stationary phase, these stains did not reflect physiological changes sufficiently and therefore additional labeling with bis-(1,3-dibutylbarbituric acid) trimethineoxonol was applied. Results of fluorescence staining in solventogenic batch fermentations were compared with substrate-use data, the concentration of key metabolites and growth curves. We demonstrate that measurements by all methods were mutually compatible.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15014581
- 003
- CZ-PrNML
- 005
- 20150423110451.0
- 007
- ta
- 008
- 150420s2014 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.anaerobe.2013.10.006 $2 doi
- 035 __
- $a (PubMed)24211310
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Patakova, Petra $u Department of Biotechnology, Institute of Chemical Technology in Prague, Technicka 5, 16628 Prague 6, Czech Republic. Electronic address: petra.patakova@vscht.cz.
- 245 10
- $a Use of fluorescent staining and flow cytometry for monitoring physiological changes in solventogenic clostridia / $c P. Patakova, M. Linhova, P. Vykydalova, B. Branska, M. Rychtera, K. Melzoch,
- 520 9_
- $a Physiological changes in populations of Clostridium beijerinckii and Clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. To estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to be a good choice for both species. During stationary phase, these stains did not reflect physiological changes sufficiently and therefore additional labeling with bis-(1,3-dibutylbarbituric acid) trimethineoxonol was applied. Results of fluorescence staining in solventogenic batch fermentations were compared with substrate-use data, the concentration of key metabolites and growth curves. We demonstrate that measurements by all methods were mutually compatible.
- 650 _2
- $a barbituráty $7 D001463
- 650 _2
- $a techniky vsádkové kultivace $7 D061249
- 650 _2
- $a bioreaktory $7 D019149
- 650 _2
- $a Clostridium $x fyziologie $x ultrastruktura $7 D003013
- 650 12
- $a fermentace $7 D005285
- 650 _2
- $a průtoková cytometrie $7 D005434
- 650 _2
- $a fluoresceiny $7 D005452
- 650 _2
- $a fluorescenční barviva $7 D005456
- 650 _2
- $a isoxazoly $7 D007555
- 650 _2
- $a propidium $7 D011419
- 650 _2
- $a barvení a značení $x metody $7 D013194
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Linhova, Michaela $u Biopreparaty spol. s r. o., Na Vinicnich horach 37, 16000 Prague 6, Czech Republic.
- 700 1_
- $a Vykydalova, Pavla $u Department of Biotechnology, Institute of Chemical Technology in Prague, Technicka 5, 16628 Prague 6, Czech Republic.
- 700 1_
- $a Branska, Barbora $u Department of Biotechnology, Institute of Chemical Technology in Prague, Technicka 5, 16628 Prague 6, Czech Republic.
- 700 1_
- $a Rychtera, Mojmir $u Department of Biotechnology, Institute of Chemical Technology in Prague, Technicka 5, 16628 Prague 6, Czech Republic.
- 700 1_
- $a Melzoch, Karel $u Department of Biotechnology, Institute of Chemical Technology in Prague, Technicka 5, 16628 Prague 6, Czech Republic.
- 773 0_
- $w MED00006567 $t Anaerobe $x 1095-8274 $g Roč. 29, č. - (2014), s. 113-7
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24211310 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150420 $b ABA008
- 991 __
- $a 20150423110751 $b ABA008
- 999 __
- $a ok $b bmc $g 1072162 $s 897459
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 29 $c - $d 113-7 $i 1095-8274 $m Anaerobe $n Anaerobe $x MED00006567
- LZP __
- $a Pubmed-20150420
