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Real-time PCR-based genotyping from whole blood using Taq DNA polymerase and a buffer supplemented with 1,2-propanediol and trehalose
P. Utekal, L. Kocanda, P. Matousek, P. Wagner, V. Bugajev, P. Draber,
Journal of immunological methods.
2015 ;
416 (-) :
178-82.
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
Perzistentní odkaz
https://www.medvik.cz/link/bmc15022966
- MeSH
- DNA chemie MeSH
- genotyp MeSH
- jednonukleotidový polymorfismus genetika MeSH
- kvantitativní polymerázová řetězová reakce metody MeSH
- lidé MeSH
- myši MeSH
- organické látky chemie MeSH
- propylenglykol chemie MeSH
- pufry MeSH
- Taq-polymerasa chemie MeSH
- trehalosa chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Amplification of DNA templates from whole blood with Taq DNA polymerase remains a difficult task worldwide. Using a real-time PCR setup and a buffer supplemented with 1M 1,2-propanediol, 0.2M trehalose, and SYBR green I we show a reliable technique for genotyping in mice and detection of single-nucleotide polymorphisms/mutations in humans. Elimination of DNA extraction and use of the common Taq DNA polymerase and DNA dye bring about substantial savings in labor and cost.
Citace poskytuje Crossref.org
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- $a Kocanda, Lukas $u Department of Signal Transduction, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, CZ-14220 Prague 4, Czech Republic.
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