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Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis
P. Podolec, AH. Szabó, J. Blaško, R. Kubinec, R. Górová, J. Višňovský, A. Gnipová, A. Horváth, V. Bierhanzl, T. Hložek, R. Čabala,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- alanin analýza chemie metabolismus MeSH
- glukosa analýza chemie metabolismus MeSH
- kyselina mléčná analýza chemie metabolismus MeSH
- limita detekce MeSH
- organické sloučeniny křemíku chemie MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody MeSH
- reprodukovatelnost výsledků MeSH
- trimethylsilylové sloučeniny chemie MeSH
- Trypanosoma brucei brucei chemie metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.
Citace poskytuje Crossref.org
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- $a Podolec, Peter $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
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- $a A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.
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- $a Szabó, Alexandra Hengerics $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
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- $a Kubinec, Róbert $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia. Electronic address: kubinec@fns.uniba.sk.
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- $a Višňovský, Jozef $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia; SynthCluster, s.r.o., Komenského 1439, 900 01 Modra, Slovakia.
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- $a Gnipová, Anna $u Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 842 15 Bratislava, Slovakia.
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- $a Bierhanzl, Václav $u Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
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- $a Čabala, Radomír $u Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic; Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
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