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Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis

P. Podolec, AH. Szabó, J. Blaško, R. Kubinec, R. Górová, J. Višňovský, A. Gnipová, A. Horváth, V. Bierhanzl, T. Hložek, R. Čabala,

. 2014 ; 967 (-) : 134-8. (Analytical technologies in the biomedical and life sciences)

Language English Country Netherlands

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc15023175

A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.

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$a Podolec, Peter $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
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$a Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis / $c P. Podolec, AH. Szabó, J. Blaško, R. Kubinec, R. Górová, J. Višňovský, A. Gnipová, A. Horváth, V. Bierhanzl, T. Hložek, R. Čabala,
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$a A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.
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$a Szabó, Alexandra Hengerics $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
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$a Blaško, Jaroslav $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
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$a Kubinec, Róbert $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia. Electronic address: kubinec@fns.uniba.sk.
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$a Górová, Renáta $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
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$a Višňovský, Jozef $u Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia; SynthCluster, s.r.o., Komenského 1439, 900 01 Modra, Slovakia.
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$a Gnipová, Anna $u Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 842 15 Bratislava, Slovakia.
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$a Horváth, Anton $u Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 842 15 Bratislava, Slovakia.
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$a Bierhanzl, Václav $u Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
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$a Hložek, Tomáš $u Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
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$a Čabala, Radomír $u Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic; Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
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