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DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I
T. Gichner, A. Mukherjee, J. Velemínský,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Alkylating Agents toxicity MeSH
- Single-Cell Analysis MeSH
- Staining and Labeling methods MeSH
- Benzoxazoles chemistry MeSH
- Quinolinium Compounds chemistry MeSH
- Deoxyribonuclease I metabolism pharmacology MeSH
- DNA, Plant analysis chemistry MeSH
- Ethidium chemistry MeSH
- Ethyl Methanesulfonate toxicity MeSH
- Fluorescent Dyes MeSH
- Indoles chemistry MeSH
- Comet Assay MeSH
- Plant Roots chemistry drug effects MeSH
- Plant Leaves chemistry drug effects MeSH
- Seedlings chemistry drug effects MeSH
- Nicotiana chemistry drug effects MeSH
- Hot Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco (Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 degrees C, and (3) DNase-I. All three treatments induced a dose-dependent increase in DNA migration, expressed as percentage of tail DNA. A comparison of the fluorochrome DNA dyes ethidium bromide, DAPI and YOYO-1 demonstrated that for the alkaline version of the comet assay in plants, the commonly used fluorescent dye ethidium bromide can be used with the same efficiency as DAPI or YOYO-1.
References provided by Crossref.org
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