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Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts
M. Khatibi Shahidi, J. Krivanek, N. Kaukua, P. Ernfors, L. Hladik, V. Kostal, S. Masich, A. Hampl, V. Chubanov, T. Gudermann, RA. Romanov, T. Harkany, I. Adameyko, K. Fried,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Ameloblasts cytology ultrastructure MeSH
- Cell Nucleus ultrastructure MeSH
- Cell Surface Extensions ultrastructure MeSH
- Dentin ultrastructure MeSH
- Extracellular Matrix ultrastructure MeSH
- Fluorescent Antibody Technique MeSH
- Ion Channels ultrastructure MeSH
- TRPM Cation Channels ultrastructure MeSH
- Cell Compartmentation MeSH
- Mesenchymal Stem Cells cytology ultrastructure MeSH
- Microscopy, Electron, Scanning methods MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Odontoblasts cytology ultrastructure MeSH
- Incisor cytology ultrastructure MeSH
- Cell Shape MeSH
- Imaging, Three-Dimensional methods MeSH
- Dental Pulp cytology ultrastructure MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In organized tissues, the precise geometry and the overall shape are critical for the specialized functions that the cells carry out. Odontoblasts are major matrix-producing cells of the tooth and have also been suggested to participate in sensory transmission. However, refined morphologic data on these important cells are limited, which hampers the analysis and understanding of their cellular functions. We took advantage of fluorescent color-coding genetic tracing to visualize and reconstruct in 3 dimensions single odontoblasts, pulp cells, and their assemblages. Our results show distinct structural features and compartments of odontoblasts at different stages of maturation, with regard to overall cellular shape, formation of the main process, orientation, and matrix deposition. We demonstrate previously unanticipated contacts between the processes of pulp cells and odontoblasts. All reported data are related to mouse incisor tooth. We also show that odontoblasts express TRPM5 and Piezo2 ion channels. Piezo2 is expressed ubiquitously, while TRPM5 is asymmetrically distributed with distinct localization to regions proximal to and within odontoblast processes.
Department of Cell and Molecular Biology Karolinska Institutet Stockholm Sweden
Department of Dental Medicine Karolinska Institutet Huddinge Sweden
Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic
Department of Neuroscience Karolinska Institutet Stockholm Sweden
References provided by Crossref.org
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