In organized tissues, the precise geometry and the overall shape are critical for the specialized functions that the cells carry out. Odontoblasts are major matrix-producing cells of the tooth and have also been suggested to participate in sensory transmission. However, refined morphologic data on these important cells are limited, which hampers the analysis and understanding of their cellular functions. We took advantage of fluorescent color-coding genetic tracing to visualize and reconstruct in 3 dimensions single odontoblasts, pulp cells, and their assemblages. Our results show distinct structural features and compartments of odontoblasts at different stages of maturation, with regard to overall cellular shape, formation of the main process, orientation, and matrix deposition. We demonstrate previously unanticipated contacts between the processes of pulp cells and odontoblasts. All reported data are related to mouse incisor tooth. We also show that odontoblasts express TRPM5 and Piezo2 ion channels. Piezo2 is expressed ubiquitously, while TRPM5 is asymmetrically distributed with distinct localization to regions proximal to and within odontoblast processes.
- MeSH
- ameloblasty cytologie ultrastruktura MeSH
- buněčné jádro ultrastruktura MeSH
- buněčné výběžky ultrastruktura MeSH
- dentin ultrastruktura MeSH
- extracelulární matrix ultrastruktura MeSH
- fluorescenční protilátková technika MeSH
- iontové kanály ultrastruktura MeSH
- kationtové kanály TRPM ultrastruktura MeSH
- kompartmentace buňky MeSH
- mezenchymální kmenové buňky cytologie ultrastruktura MeSH
- mikroskopie elektronová rastrovací metody MeSH
- myši transgenní MeSH
- myši MeSH
- odontoblasty cytologie ultrastruktura MeSH
- řezáky cytologie ultrastruktura MeSH
- tvar buňky MeSH
- zobrazování trojrozměrné metody MeSH
- zubní dřeň cytologie ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
