• Je něco špatně v tomto záznamu ?

In vitro culturing of viable circulating tumor cells of urinary bladder cancer

M. Cegan, K. Kolostova, R. Matkowski, M. Broul, J. Schraml, M. Fiutowski, V. Bobek,

. 2014 ; 7 (10) : 7164-71. [pub] 20140915

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15031725

OBJECTIVE: Approximately one third of patients diagnosed with muscle-invasive urinary bladder cancer (UBC) have undetected metastases at the time of treatment of the primary tumor. Currently there are no reliable specific serum markers for monitoring and evaluating risk profiles of urothelial cancers. Several studies suggest that detection of circulating tumor cells (CTCs) may correlate with the disease status and prognosis at baseline and early in the treatment of cancers. In this study a new way of isolation and in vitro cultivation of CTCs of urinary bladder cancer was introduced. MATERIALS AND METHODS: Peripheral blood (PB) samples from 53 patients who had undergone urological procedure were evaluated using the MetaCell device (MetaCell s.r.o., Ostrava, Czech Republic). The patients enrolled in the study were both oncological patients with UBC and non-oncological patients with inflammation (14 patients). The sensitivity and quantification of CTCs were evaluated. The separated CTCs were cultured in vitro. RESULTS: 39 patients with confirmed UBC were enrolled in the study. CTCs were detected in 25 (64%) patients, and most of these patients had between 6 and 10 cells. The separated CTCs were successfully cultured in vitro. CONCLUSION: CTCs were detected in a higher percentage of patients than in other studies. This paper describes the first successful culturing of human UBC cells. The MetaCell approach used in this study enabled the capture of viable intact virgin CTCs (virgin CTC) suitable for next in vitro culturing, single cell analysis or drug testing.

000      
00000naa a2200000 a 4500
001      
bmc15031725
003      
CZ-PrNML
005      
20160610122600.0
007      
ta
008      
151005s2014 xxu f 000 0|eng||
009      
AR
035    __
$a (PubMed)25400813
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Cegan, Martin $u Department of Pathology, Masaryk's Hospital in Usti Nad Labem Krajska Zdravotni a.s., Socialni Pece 3316, 12A40113 Usti Nad Labem, Czech Republic.
245    10
$a In vitro culturing of viable circulating tumor cells of urinary bladder cancer / $c M. Cegan, K. Kolostova, R. Matkowski, M. Broul, J. Schraml, M. Fiutowski, V. Bobek,
520    9_
$a OBJECTIVE: Approximately one third of patients diagnosed with muscle-invasive urinary bladder cancer (UBC) have undetected metastases at the time of treatment of the primary tumor. Currently there are no reliable specific serum markers for monitoring and evaluating risk profiles of urothelial cancers. Several studies suggest that detection of circulating tumor cells (CTCs) may correlate with the disease status and prognosis at baseline and early in the treatment of cancers. In this study a new way of isolation and in vitro cultivation of CTCs of urinary bladder cancer was introduced. MATERIALS AND METHODS: Peripheral blood (PB) samples from 53 patients who had undergone urological procedure were evaluated using the MetaCell device (MetaCell s.r.o., Ostrava, Czech Republic). The patients enrolled in the study were both oncological patients with UBC and non-oncological patients with inflammation (14 patients). The sensitivity and quantification of CTCs were evaluated. The separated CTCs were cultured in vitro. RESULTS: 39 patients with confirmed UBC were enrolled in the study. CTCs were detected in 25 (64%) patients, and most of these patients had between 6 and 10 cells. The separated CTCs were successfully cultured in vitro. CONCLUSION: CTCs were detected in a higher percentage of patients than in other studies. This paper describes the first successful culturing of human UBC cells. The MetaCell approach used in this study enabled the capture of viable intact virgin CTCs (virgin CTC) suitable for next in vitro culturing, single cell analysis or drug testing.
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a papilární karcinom $x krev $x patologie $7 D002291
650    _2
$a studie případů a kontrol $7 D016022
650    _2
$a proliferace buněk $7 D049109
650    _2
$a separace buněk $x metody $7 D002469
650    _2
$a viabilita buněk $7 D002470
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a stupeň nádoru $7 D060787
650    _2
$a staging nádorů $7 D009367
650    _2
$a nádorové cirkulující buňky $x patologie $7 D009360
650    _2
$a nádorové buňky kultivované $7 D014407
650    _2
$a nádory močového měchýře $x krev $x patologie $7 D001749
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kolostova, Katarina $u Department of Laboratory Genetics University Hospital Kralovske Vinohrady Srobarova 50, 100 97 Prague, Czech Republic.
700    1_
$a Matkowski, Rafal $u Department of Oncology and Division of Oncological Surgery, Wroclaw Medical University ul. Pasteura 1, 50-367 Wrocław, Poland ; Lower Silesian Oncology Centre Plac Hirszfelda 12, 53-413 Wrocław, Poland.
700    1_
$a Broul, Marek $u Department of Urology and Center of Robotic Surgery, Masaryk's Hospital in Usti Nad Labem Krajska Zdravotni a.s., Socialni Pece 3316, 12A40113 Usti Nad Labem, Czech Republic. $7 xx0117935
700    1_
$a Schraml, Jan $u Department of Urology and Center of Robotic Surgery, Masaryk's Hospital in Usti Nad Labem Krajska Zdravotni a.s., Socialni Pece 3316, 12A40113 Usti Nad Labem, Czech Republic.
700    1_
$a Fiutowski, Marek $u Department of Urology, Regional Specialistic Hospital ul. Kamienskiego 73a, 51-318 Wroclaw, Poland.
700    1_
$a Bobek, Vladimir $u Department of Laboratory Genetics University Hospital Kralovske Vinohrady Srobarova 50, 100 97 Prague, Czech Republic ; Department of Histology and Embryology, Wroclaw Medical University Chalubinskiego 6a, 50-368 Wroclaw, Poland ; Third Department of Surgery, First Faculty of Medicine, Charles University in Prague and University Hospital Motol 105 00 Prague, Czech Republic.
773    0_
$w MED00165429 $t International journal of clinical and experimental pathology $x 1936-2625 $g Roč. 7, č. 10 (2014), s. 7164-71
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25400813 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20151005 $b ABA008
991    __
$a 20160610122728 $b ABA008
999    __
$a ok $b bmc $g 1092601 $s 914851
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 7 $c 10 $d 7164-71 $e 20140915 $i 1936-2625 $m International journal of clinical and experimental pathology $n Int. j. clin. exp. pathol. $x MED00165429
LZP    __
$a Pubmed-20151005

Najít záznam

Citační ukazatele

Možnosti archivace